| Automated quantitative determination of a new polyamine biosynthesis inhibitor (CGP 48 664) and a potential metabolite in human and animal plasma by high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 8811445 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A specific and sensitive liquid chromatographic assay for the determination of 4-amidino-1-indanone-2'-amidinohydrazone (CGP 48 664, I) and a potential metabolite, 2-(4-carbamoyl-2,3-dihydro-1H-inden-1-yliden) hydrazine carboximidamide (CGP 53 391, II), in human and animal plasma was developed. CGP 51 467, a structural analog, was added to the plasma samples (up to 1 ml) as an internal standard. After mixing, the samples were processed automatically using an ASPEC solid-phase extraction system. The final extracts were chromatographed on a 5 microns Purospher RP-18 HPLC column. Chromatography was performed using a gradient system and UV detection. The described HPLC method is suitable for specific and quantitative measurement of concentrations of I, as well as its potential metabolite II down to 5-10 ng/ml in human and animal (dog, rat) plasma with acceptable reproducibility and accuracy. |
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Authors:
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P H Degen; P Zbinden |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical applications Volume: 681 ISSN: 1572-6495 ISO Abbreviation: J. Chromatogr. B, Biomed. Appl. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-12-10 Completed Date: 1996-12-10 Revised Date: 2007-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9421796 Medline TA: J Chromatogr B Biomed Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 339-45 Citation Subset: IM |
Affiliation:
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Research and Development Department, Ciba-Geigy Limited, Basel, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosylmethionine Decarboxylase
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antagonists & inhibitors* Amidines / blood*, pharmacokinetics Animals Autoanalysis* Chromatography, High Pressure Liquid / methods*, statistics & numerical data Dogs Drug Stability Enzyme Inhibitors / blood* Guanidines / blood Humans Hydrazones / blood Indans / blood*, pharmacokinetics Rats Regression Analysis Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Amidines; 0/Enzyme Inhibitors; 0/Guanidines; 0/Hydrazones; 0/Indans; 149400-88-4/4-amidinoindan-1-one 2'-amidinohydrazone; 179528-40-6/CGP 53391; EC 4.1.1.50/Adenosylmethionine Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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