Document Detail

Automated quantitation of hemoglobin-based blood substitutes in whole blood samples.
MedLine Citation:
PMID:  11764082     Owner:  NLM     Status:  MEDLINE    
It is necessary to develop methods for accurate monitoring of cell-free hemoglobin in circulation. Routine monitoring of circulating cell-free hemoglobin will be useful for evaluating the efficacy of blood substitute administration andfor determining the clearance rates of the blood substitute from circulation. In addition, discriminating between cell-free hemoglobin and cell-associated hemoglobin will enable accurate determination of RBC indices, mean cell hemoglobin and mean corpuscular hemoglobin concentration, in individuals receiving hemoglobin-based blood substitutes. As colorimetric methods used by hematology analyzers to quantitate the hemoglobin value of a blood sample cannot distinguish between cell-associated and cell-free hemoglobin, it is currently not feasible to quantitate the levels of hemoglobin substitutes in circulation. The advent of a technology that measures volume and hemoglobin concentration of individual RBCs provides an alternative strategy for quantitating the cell-associated hemoglobin in a blood sample. We document that the combined use of cell-based and colorimetric hemoglobin measurements provides accurate discrimination between cell-associated and cell-free hemoglobin over a wide range of hemoglobin levels. This strategy should enable rapid and accurate monitoring of the levels of cell-free hemoglobin substitutes in the circulation of recipients of these blood substitutes.
J Kunicka; M Malin; D Zelmanovic; M Katzenberg; W Canfield; P Shapiro; N Mohandas
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  116     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-14     Completed Date:  2002-01-03     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  913-9     Citation Subset:  AIM; IM    
Laboratory Testing Segment, Bayer, Tarrytown, NY, USA.
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MeSH Terms
Anemia, Iron-Deficiency / blood
Anemia, Sickle Cell / blood
Blood Substitutes / analysis*
Erythrocytes / chemistry*
Hemoglobinometry / methods*
Hemoglobins / analysis*
Reproducibility of Results
Scattering, Radiation
Thalassemia / blood
Reg. No./Substance:
0/Blood Substitutes; 0/Hemoglobins

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