| Automated quantitation of hemoglobin-based blood substitutes in whole blood samples. | |
MedLine Citation:
|
PMID: 11764082 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
It is necessary to develop methods for accurate monitoring of cell-free hemoglobin in circulation. Routine monitoring of circulating cell-free hemoglobin will be useful for evaluating the efficacy of blood substitute administration andfor determining the clearance rates of the blood substitute from circulation. In addition, discriminating between cell-free hemoglobin and cell-associated hemoglobin will enable accurate determination of RBC indices, mean cell hemoglobin and mean corpuscular hemoglobin concentration, in individuals receiving hemoglobin-based blood substitutes. As colorimetric methods used by hematology analyzers to quantitate the hemoglobin value of a blood sample cannot distinguish between cell-associated and cell-free hemoglobin, it is currently not feasible to quantitate the levels of hemoglobin substitutes in circulation. The advent of a technology that measures volume and hemoglobin concentration of individual RBCs provides an alternative strategy for quantitating the cell-associated hemoglobin in a blood sample. We document that the combined use of cell-based and colorimetric hemoglobin measurements provides accurate discrimination between cell-associated and cell-free hemoglobin over a wide range of hemoglobin levels. This strategy should enable rapid and accurate monitoring of the levels of cell-free hemoglobin substitutes in the circulation of recipients of these blood substitutes. |
Authors:
|
J Kunicka; M Malin; D Zelmanovic; M Katzenberg; W Canfield; P Shapiro; N Mohandas |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: American journal of clinical pathology Volume: 116 ISSN: 0002-9173 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2001 Dec |
Date Detail:
|
Created Date: 2001-12-14 Completed Date: 2002-01-03 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
|
Languages: eng Pagination: 913-9 Citation Subset: AIM; IM |
Affiliation:
|
Laboratory Testing Segment, Bayer, Tarrytown, NY, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anemia, Iron-Deficiency
/
blood Anemia, Sickle Cell / blood Blood Substitutes / analysis* Erythrocytes / chemistry* Hemoglobinometry / methods* Hemoglobins / analysis* Humans Light Reproducibility of Results Scattering, Radiation Thalassemia / blood |
| Chemical | |
Reg. No./Substance:
|
0/Blood Substitutes; 0/Hemoglobins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Laboratory, morphologic, and immunophenotypic correlates of surface immunoglobulin heavy chain isoty...
Next Document: Regulation of Th1 and Th2 lymphocyte migration by human adult brain endothelial cells.