Document Detail


Automated purification of synthetic oligonucleotides.
MedLine Citation:
PMID:  1878202     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have automated the trityl-on purification of oligonucleotides by use of an XYZ axis robotic solid-phase extraction system. This greatly decreased the preparation time required for oligonucleotide purification. After about 15 min for set up of the samples and instrument, the oligonucleotides are automatically purified with a 15-min run time per sample. Thus, for example, the purification of 15 oligonucleotides requires only about 15 min of preparation time and 4 h of machine time. Yields and purity are equivalent to manual methods.
Authors:
K M Ivanetich; J Akiyama; D V Santi; M Reschenberg
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BioTechniques     Volume:  10     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  704-8     Citation Subset:  IM    
Affiliation:
University of California, San Francisco 94143-0448.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Chromatography / instrumentation*
Chromatography, High Pressure Liquid
Methods
Oligonucleotides / chemical synthesis,  isolation & purification*
Renin
Trityl Compounds
Chemical
Reg. No./Substance:
0/Oligonucleotides; 0/Trityl Compounds; EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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