Document Detail

Automated multiple-cell karyotyping: a clinical feasibility study.
MedLine Citation:
PMID:  1860250     Owner:  NLM     Status:  MEDLINE    
In order to increase the efficiency of the Magiscan metaphase location and karyotyping system, its software and mode of operation have been changed. In the new multiple-cell karyotyping method, interactions by the operator are only required for relocation and counting of metaphases, but not for karyotyping. Metaphases are located and their coordinates recorded automatically as before. The first metaphase in the list is relocated, displayed on the screen, and counted by the operator. It is then karyotyped automatically while the operator relocates and counts the next metaphase in the list. This procedure continues until an appropriate number of metaphases have been counted and karyotyped. Finally a composite karyotype is printed out. Each karyotype is represented by a column of 23 chromosome pairs (1-22 and XX or XY) and all columns are lined up next to each other. Most chromosomes are correctly classified into the composite karyotype. Minor structural abnormalities are detected by comparing pairs of homologues. Overlapped, close touching, and grossly abnormal chromosomes are often misclassified or rejected and shown beneath the classified chromosomes. A trained cytotechnician can easily detect even small chromosome abnormalities on the composite karyotype. A clinical feasibility study indicates that the procedure can be used for routine cytogenetic analysis.
C Lundsteen; T Gerdes; J Maahr
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical genetics     Volume:  39     ISSN:  0009-9163     ISO Abbreviation:  Clin. Genet.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-09-04     Completed Date:  1991-09-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0253664     Medline TA:  Clin Genet     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  338-46     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, Rigshospitalet, Copenhagen, Denmark.
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MeSH Terms
Amniotic Fluid / cytology*
Chromosome Aberrations / diagnosis*,  genetics
Chromosome Disorders
Image Processing, Computer-Assisted / instrumentation*
Karyotyping / instrumentation*
Prenatal Diagnosis

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