Document Detail

Automated metaphase finding: an assessment of the efficiency of the METAFER2 system in a routine mutagenicity assay.
MedLine Citation:
PMID:  7528343     Owner:  NLM     Status:  MEDLINE    
The efficiency of the automated metaphase finding system METAFER2 is assessed in a routine mutagenicity assay using an aneuploid rat liver cell line treated with various promutagens. Data sets generated by automated and manual selection of metaphases are compared. It is demonstrated that METAFER2 routinely allows an efficient automatic identification of metaphases not only in lymphocyte preparations, but also in preparations from mammalian cell lines with varying chromosome numbers. Although larger slide areas are required for automated compared to manual metaphase scanning, the automatic system is faster by a factor of about 5. The interactive visual elimination of metaphases of insufficient quality is an easy and fast procedure. METAFER2 allows an unbiased selection of metaphases irrespective of their appearance as homogeneously stained first or harlequin-stained second division cells. Random selection of metaphases is neither influenced by various structural chromosome changes nor by increased frequencies of sister-chromatid exchanges.
R Huber; U Kulka; T Lörch; H Braselmann; M Bauchinger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  334     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-01-20     Completed Date:  1995-01-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  97-102     Citation Subset:  IM    
GSF-Forschungszentrum für Umwelt und Gesundheit, Institute of Radiobiology, Oberschleissheim/Neuherberg, Germany.
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MeSH Terms
Cell Line
Chromosome Aberrations
Evaluation Studies as Topic
Image Processing, Computer-Assisted*
Mutagenicity Tests / methods*
Mutagens / toxicity
Sister Chromatid Exchange
Time Factors
Reg. No./Substance:

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