Document Detail


Automated measurement of spatial preference in the open field test with transmitted lighting.
MedLine Citation:
PMID:  18342949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
New modification of the open field was designed to improve automation of the test. The main innovations were: (1) transmitted lighting and (2) estimation of probability to find pixels associated with an animal in the selected region of arena as an objective index of spatial preference. Transmitted (inverted) lighting significantly ameliorated the contrast between an animal and arena and allowed to track white animals with similar efficacy as colored ones. Probability as a measure of preference of selected region was mathematically proved and experimentally verified. A good correlation between probability and classic indices of spatial preference (number of region entries and time spent therein) was shown. The algorithm of calculation of probability to find pixels associated with an animal in the selected region was implemented in the EthoStudio software. Significant interstrain differences in locomotion and the central zone preference (index of anxiety) were shown using the inverted lighting and the EthoStudio software in mice of six inbred strains. The effects of arena shape (circle or square) and a novel object presence in the center of arena on the open field behavior in mice were studied.
Authors:
Alexander V Kulikov; Maria A Tikhonova; Victor A Kulikov
Related Documents :
7941399 - The influence of contrast adaptation on color appearance.
15518209 - Information limits on neural identification of colored surfaces in natural scenes.
10600149 - Does darkening signal submission in territorial contests between juvenile atlantic salm...
1455719 - Fourier analysis of the stimuli for pattern-induced flicker colors.
8492959 - Color perception profiles in central achromatopsia.
1233329 - Prehatch color stimulation effects on color pecking preferences and color discriminatio...
24019959 - Proprioception is robust under external forces.
15363669 - The topology of defibrillation.
17507389 - Phototropins and neochrome1 mediate nuclear movement in the fern adiantum capillus-vene...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-07
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  170     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-18     Completed Date:  2008-07-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  345-51     Citation Subset:  IM    
Affiliation:
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Avenue Lavrentyev 10, Novosibirsk 630090, Russia. akulikov@ngs.ru
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Automation
Behavior, Animal / physiology*
Lighting
Male
Mice
Mice, Inbred A
Mice, Inbred AKR
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Inbred DBA
Software
Space Perception / physiology*
Species Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  How do mutant Nav1.1 sodium channels cause epilepsy?
Next Document:  Automated detection and analysis of foraging behavior in Caenorhabditis elegans.