Document Detail


Automated liquid microjunction surface sampling-HPLC-MS/MS analysis of drugs and metabolites in whole-body thin tissue sections.
MedLine Citation:
PMID:  23534426     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background: The aim of this work was to develop a fully automated liquid extraction-based surface sampling system utilizing a commercially available autosampler coupled with HPLC-MS/MS detection. Results: Discrete spots selected for droplet-based sampling and automated sample queue generation, for both the autosampler and MS, were enabled by using in-house developed software. In addition, co-registration of spatially resolved sampling positions and HPLC-MS information to generate heat maps of compounds monitored for subsequent data analysis was also available in the software. The system was evaluated with whole-body thin tissue sections from propranolol-dosed rats. Conclusion: The spatial distributions of both the drug and its hydroxypropranolol glucuronide metabolites were consistent with previous studies employing other liquid extraction-based surface sampling methodologies.
Authors:
Vilmos Kertesz; Gary J Van Berkel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioanalysis     Volume:  5     ISSN:  1757-6199     ISO Abbreviation:  Bioanalysis     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101512484     Medline TA:  Bioanalysis     Country:  England    
Other Details:
Languages:  eng     Pagination:  819-26     Citation Subset:  IM    
Affiliation:
Organic & Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA.
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