Document Detail


Automated image analysis of micronuclei by IMSTAR for biomonitoring.
MedLine Citation:
PMID:  21164198     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
For many years, the analysis of micronuclei (MN) has been successfully applied to human biomonitoring of in vivo genotoxin exposure and provides a sensitive and relatively easy methodology to assess genomic instability. However, there is a need for automation of MN analysis for rapid, more reliable and non-subjective MN detection. In this review, we evaluate the application of automated image analysis of the in vitro cytokinesis-block MN assay on human lymphocytes for human biomonitoring, starting with the requirements that should be fulfilled by a valid and efficient image analysis system. Considering these prerequisites, we compare the automated facility developed in the framework of the European Union-project NewGeneris with other already published systems for automated scoring of MN. Although the automated scoring of MN is now put into place, extension to other cytome assay end points such as apoptosis, necrosis, nuclear buds and nucleoplasmic bridges would greatly enhance the specificity and sensitivity of future biomonitoring studies. Inclusion of these end points would also allow an automated approach to in vitro genotoxicity testing. In addition, automated scoring of the MN assay in exfoliated buccal cells would be very beneficial for large-scale biomonitoring studies, as cells can be collected in a minimally invasive manner.
Authors:
Ilse Decordier; Alexandre Papine; Kim Vande Loock; Gina Plas; Françoise Soussaline; Micheline Kirsch-Volders
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutagenesis     Volume:  26     ISSN:  1464-3804     ISO Abbreviation:  Mutagenesis     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8707812     Medline TA:  Mutagenesis     Country:  England    
Other Details:
Languages:  eng     Pagination:  163-8     Citation Subset:  IM    
Affiliation:
Laboratorium voor Cellulaire Genetica, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium. idecordi@vub.ac.be
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