| Automated fluorescent in situ hybridization (FISH) analysis of t(9;22)(q34;q11) in interphase nuclei. | |
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MedLine Citation:
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PMID: 16646048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: For chronic myeloid leukemia, the FISH detection of t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes is an alternative method to bone marrow karyotyping for monitoring treatment. With automation, several drawbacks of manual analysis may be circumvented. In this article, the capabilities of a commercially available automated image acquisition and analysis system were determined by detecting t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes. METHODS: Three peripheral blood samples of normal adults, 21 samples of CML patients, and one sample of a t(9;22)(q34;q11) positive cell-line were used. RESULTS: Single nuclei with correctly detected signals amounted to 99.6% of nuclei analyzed after exclusion of overlapping nuclei and nuclei with incorrect signal detection. A cut-off value of 0.84 mum was defined to discriminate between translocation positive and negative nuclei based on the shortest distance between signals. Using this value, the false positive rate of the automated analysis for negative samples was 7.0%, whereas that of the manual analysis was 5.8%. Automated and manual results showed strong correlation (R(2) = 0.985), the mean difference of results was only 3.7%. CONCLUSIONS: A reliable and objective automated analysis of large numbers of cells is possible, avoiding interobserver variability and producing statistically more accurate results than manual evaluation. |
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Authors:
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Béla Kajtár; Gábor Méhes; Thomas Lörch; Linda Deák; Marika Kneifné; Donát Alpár; László Pajor |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytometry. Part A : the journal of the International Society for Analytical Cytology Volume: 69 ISSN: 1552-4922 ISO Abbreviation: Cytometry A Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-29 Completed Date: 2006-07-31 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101235694 Medline TA: Cytometry A Country: United States |
Other Details:
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Languages: eng Pagination: 506-14 Citation Subset: IM |
Copyright Information:
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Copyright 2006 International Society for Analytical Cytology. |
Affiliation:
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Department of Pathology, Faculty of Medicine, University of Pécs, Hungary. bkajtar@pathology.pote.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood Cells / chemistry Cell Nucleus / chemistry* Chromosomes, Human, Pair 22 Chromosomes, Human, Pair 9 False Positive Reactions Humans In Situ Hybridization, Fluorescence / methods* Interphase / genetics Karyotyping Leukemia, Myelogenous, Chronic, BCR-ABL Positive / diagnosis*, genetics Philadelphia Chromosome* Signal Processing, Computer-Assisted Translocation, Genetic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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