| Automated extraction and classification of dynamic metrical features of morphological development in dissociated Purkinje neurons. | |
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MedLine Citation:
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PMID: 19837112 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The morphological development of in vitro single cerebellar Purkinje cells obtained from wild type P1 CD1 mice was assessed through a dedicated non-invasive technique based on image processing algorithms and multivariate analysis. Image processing algorithms were implemented to extract metrical features characterizing cell structure and dendritic arborization from sequential optical micrographs. Quantitative morphological features were analyzed in order to identify relevant metrical characteristics common to Purkinje cells in wild type P1 CD1 mice. Cell arborization was found to be characterized by a high fractal dimension and the directionality and level of complexity were shown to be key features for cell morphology classification, as underlined using a three-way PCA analysis. |
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Authors:
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L Billeci; G Pioggia; F Vaglini; A Ahluwalia |
Publication Detail:
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Type: Journal Article Date: 2009-10-28 |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 185 ISSN: 1872-678X ISO Abbreviation: J. Neurosci. Methods Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 315-24 Citation Subset: IM |
Affiliation:
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Interdepartmental Research Center E. Piaggio, University of Pisa, via Diotisalvi 2, 56126 Pisa, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Cerebellum / cytology* Linear Models Mice Multivariate Analysis Nonlinear Dynamics* Principal Component Analysis Purkinje Cells / cytology*, physiology Radiographic Image Interpretation, Computer-Assisted / methods* Signal Processing, Computer-Assisted* Time Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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