Document Detail


Automated electrochemical free radical scavenger screening in dietary samples.
MedLine Citation:
PMID:  22881402     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
An automated electrochemical microtiter plate assay for the quantification of free radical scavengers (antioxidants) in food samples is described. Dietary antioxidant capacity measurements were achieved using the radical compound 2,2-diphenyl-1-picrylhydrazyl (DPPH·) as an amperometric redox indicator, with a pencil lead working electrode, in conjunction with a Pt counter-electrode and Ag/AgCl reference electrode, moving sequentially through the 24 vials of a standard 6 × 4 microtiter plate. Programmed analyses were performed successfully with synthetic antioxidants of known concentrations and with tea infusions, fruit juices, and vegetable extracts. The novel methodology is simple and convenient as, unlike common DPPH· antioxidant electroanalysis, it avoids the time-consuming cleaning of electrochemical cells between measurements. Microtiter plate-based robotic electrochemical antioxidant assaying is thus a good option for handling large sample collections and may be applicable in the production of food and herbal remedies.
Authors:
Sireerat Intarakamhang; Albert Schulte
Publication Detail:
Type:  Journal Article     Date:  2012-07-26
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6767-74     Citation Subset:  IM    
Affiliation:
Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Biochemistry, Institute of Science, Suranaree University of Technology , Nakhon Ratchasima 30000, Thailand.
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