Document Detail


Automated dried blood spots standard and QC sample preparation using a robotic liquid handler.
MedLine Citation:
PMID:  23216120     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background: A dried blood spot (DBS) bioanalysis assay involves many steps, such as the preparation of standard (STD) and QC samples in blood, the spotting onto DBS cards, and the cutting-out of the spots. These steps are labor intensive and time consuming if done manually, which, therefore, makes automation very desirable in DBS bioanalysis. Results: A robotic liquid handler was successfully applied to the preparation of STD and QC samples in blood and to spot the blood samples onto DBS cards using buspirone as the model compound. This automated preparation was demonstrated to be accurate and consistent. However the accuracy and precision of automated preparation were similar to those from manual preparation. The effect of spotting volume on accuracy was evaluated and a trend of increasing concentrations of buspirone with increasing spotting volumes was observed. Conclusion: The automated STD and QC sample preparation process significantly improved the efficiency, robustness and safety of DBS bioanalysis.
Authors:
Long Yuan; Duxi Zhang; Anne-Francoise Aubry; Mark E Arnold
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioanalysis     Volume:  4     ISSN:  1757-6199     ISO Abbreviation:  Bioanalysis     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101512484     Medline TA:  Bioanalysis     Country:  England    
Other Details:
Languages:  eng     Pagination:  2795-804     Citation Subset:  IM    
Affiliation:
Bioanalytical Sciences, Bristol-Myers Squibb, Route 206 & Province Line Road, Princeton, NJ 08543, USA.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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