| Automated counting of mammalian cell colonies. | |
| | |
MedLine Citation:
|
PMID: 11197679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Investigating the effect of low-dose radiation exposure on cells using assays of colony-forming ability requires large cell samples to maintain statistical accuracy. Manually counting the resulting colonies is a laborious task in which consistent objectivity is hard to achieve. This is true especially with some mammalian cell lines which form poorly defined or 'fuzzy' colonies, typified by glioma or fibroblast cell lines. A computer-vision-based automated colony counter is presented in this paper. It utilizes novel imaging and image-processing methods involving a modified form of the Hough transform. The automated counter is able to identify less-discrete cell colonies typical of these cell lines. The results of automated colony counting are compared with those from four manual (human) colony counts for the cell lines HT29, A172, U118 and IN1265. The results from the automated counts fall well within the distribution of the manual counts for all four cell lines with respect to surviving fraction (SF) versus dose curves, SF values at 2 Gy (SF2) and total area under the SF curve (Dbar). From the variation in the counts, it is shown that the automated counts are generally more consistent than the manual counts. |
| | |
Authors:
|
P R Barber; B Vojnovic; J Kelly; C R Mayes; P Boulton; M Woodcock; M C Joiner |
Related Documents
:
|
1507189 - Fetal cells in the maternal circulation. 44949 - Quantitative determination of the disintegration of nerve cells in the cortex caused by... 21181989 - Single-cell tracking with a reversing flow cytometer. 11333329 - Identifying the origin of single corneal cells by dna fingerprinting: part i--implicati... 3959069 - A sample preparation for quantitative determination of magnesium in individual lymphocy... 15086109 - Comparison of tracheal aspirates and bronchoalveolar lavage in racehorses. 1. evaluatio... 21798289 - Fluoroquinolones inhibit human polyomavirus bk (bkv) replication in primary human kidne... 16983559 - Biological treatment strategies for disc degeneration: potentials and shortcomings. 20810259 - 3d structural and functional characterization of the transition from hodgkin to reed-st... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Physics in medicine and biology Volume: 46 ISSN: 0031-9155 ISO Abbreviation: Phys Med Biol Publication Date: 2001 Jan |
Date Detail:
|
Created Date: 2001-01-24 Completed Date: 2001-03-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0401220 Medline TA: Phys Med Biol Country: England |
Other Details:
|
Languages: eng Pagination: 63-76 Citation Subset: IM |
Affiliation:
|
Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, UK. barber@graylab.ac.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Automation* Cell Count* Cell Line Cell Separation Cell Survival Cells, Cultured / radiation effects* Cytological Techniques / methods* Dose-Response Relationship, Radiation Fibroblasts / cytology Flow Cytometry Humans Image Processing, Computer-Assisted / methods Reproducibility of Results Software Tumor Cells, Cultured |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Non-invasive and quantitative near-infrared haemoglobin spectrometry in the piglet brain during hypo...
Next Document: Evaluation of inverse methods and head models for EEG source localization using a human skull phanto...