Document Detail

Automated cerebrospinal fluid cytology: limitations and reasonable applications.
MedLine Citation:
PMID:  16121639     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the precision and clinical applicability of the Bayer ADVIA 120 cytometer (Bayer Healthcare, Fernwald, Germany) for cerebrospinal fluid (CSF) cell count and differentiation.
STUDY DESIGN: One hundred six analyses of CSF from 98 patients by the ADVIA 120 were compared with routine cell count and microscopic differentiation. Correlation coefficients were calculated.
RESULTS: In general, the total cell counts of both methods correlated well. The best correlations were seen at higher cell counts, > or = 100 cells per microliter with < 100 erythrocytes per microliter. The best correlations of cell differentiation were seen for lymphocytes and neutrophils, while the results for monocytes and eosinophils were less precise. In some cases, considerable differences between automated and microscopic cell counts and differentiation were seen that were relevant to clinical decision making. The detection of pathologic cell types, such as hemosiderophages, mitoses and neoplastic cells, was not provided by automated cytometry.
CONCLUSION: When experienced personnel are not available, a preliminary cell count and differentiation between neutrophilic and lymphocytic reactions by automated cytometry may be valuable in allowing initial therapeutic decision making. Since the detection of pathologic cell types is not provided and the precision at low cell counts is only moderate, a personal microscopic evaluation of each sample is still indispensable to avoid misdiagnoses.
Herwig Strik; Hilmar Luthe; Ingelore Nagel; Bettina Ehrlich; Mathias Bahr
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  27     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-08-26     Completed Date:  2005-10-20     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  167-73     Citation Subset:  IM    
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MeSH Terms
Cell Count / instrumentation,  methods
Central Nervous System / pathology
Cerebrospinal Fluid / cytology*
Erythrocytes / cytology
False Negative Reactions
False Positive Reactions
Flow Cytometry / instrumentation*,  methods
Granulocytes / cytology
Leukocyte Count
Linear Models
Lymphocytes / cytology
Lymphoma / cerebrospinal fluid,  pathology
Monocytes / cytology
Plasmacytoma / cerebrospinal fluid,  pathology
Pneumococcal Infections / cerebrospinal fluid,  pathology
Sensitivity and Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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