| Automated RFLP pattern comparison and similarity coefficient calculation for rapid delineation of new and distinct phytoplasma 16Sr subgroup lineages. | |
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MedLine Citation:
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PMID: 18842858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phytoplasmas are cell wall-less bacteria that cause numerous diseases in several hundred plant species. During adaptation to transkingdom parasitism in diverse plant and insect hosts, phytoplasma evolution has given rise to widely divergent lineages. Since phytoplasmas cannot be cultured in a cell-free medium, measurable phenotypic characters suitable for conventional microbial classification are mostly inaccessible. Currently, phytoplasma differentiation and classification are mainly dependent on restriction fragment length polymorphism (RFLP) analysis of 16S rRNA gene sequences. Extending our recent efforts in the exploitation of computer-simulated 16S rRNA gene RFLP analysis and virtual gel plotting for rapid classification of phytoplasmas, we have developed a Perl program for automated RFLP pattern comparison and similarity coefficient calculation. This program streamlines virtual RFLP pattern analysis and has led to the establishment of a criterion for phytoplasma 16Sr subgroup classification and to the delineation of new and distinct subgroup lineages in the clover proliferation phytoplasma group (16SrVI). |
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Authors:
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Wei Wei; Ing-Ming Lee; Robert E Davis; Xiaobing Suo; Yan Zhao |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: International journal of systematic and evolutionary microbiology Volume: 58 ISSN: 1466-5026 ISO Abbreviation: Int. J. Syst. Evol. Microbiol. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-09 Completed Date: 2008-11-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100899600 Medline TA: Int J Syst Evol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 2368-77 Citation Subset: IM |
Affiliation:
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Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Typing Techniques Computer Simulation DNA Restriction Enzymes / chemistry DNA, Bacterial / genetics Genes, Bacterial Genes, rRNA Medicago / microbiology Pattern Recognition, Automated Phylogeny Phytoplasma / classification*, enzymology, genetics* Plant Diseases / microbiology Polymorphism, Restriction Fragment Length* RNA, Ribosomal, 16S / genetics* Sequence Analysis, DNA Software* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/RNA, Ribosomal, 16S; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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