| Automated Protein Hydrolysis Delivering Sample to a Solid Acid Catalyst for Amino Acid Analysis. | |
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MedLine Citation:
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PMID: 20923146 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this study, we developed an automatic protein hydrolysis system using strong cation-exchange resins as solid acid catalysts. Examining several kinds of inorganic solid acids and cation-exchange resins, we found that a few cation-exchange resins worked as acid catalysts for protein hydrolysis when heated in the presence of water. The most efficient resin yielded amounts of amino acids that were over 70% of those recovered after conventional hydrolysis with hydrochloric acid and resulted in amino acid compositions matching the theoretical values. The solid-acid hydrolysis was automated by packing the resin into columns, combining the columns with a high-performance liquid chromatography system, and heating them. The amino acids that constitute a protein can thereby be determined, minimizing contamination from the environment. |
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Authors:
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Akiko Masuda; Naoshi Dohmae |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-10-5 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Biomolecular Characterization Team, Riken Advanced Science Institute, and CREST, JST, 2-1 Hirosawa, Wako, Saitama 351-0198. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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