Document Detail

Autologous urothelial cells transplantation onto a prefabricated capsular stent for tissue engineered ureteral reconstruction.
MedLine Citation:
PMID:  22382733     Owner:  NLM     Status:  Publisher    
In this study, we have fabricated an artificial ureter by transplantation of in vitro-expanded urothelial cells onto an in vivo-prefabricated capsular stent using tissue engineering methods. Spiral poly (L: -lactic acid) (PLLA) stents were transplanted into the subcutaneous of Wistar rats for a period of 1, 2 or 3 weeks to induce the formation of connective tissue capsules on their surfaces. The capsular PLLA stents were then decellularized and further recellularized with bladder epithelial cells to fabricate artificial ureters. The results showed that the entrapped cells in all capsules remained continuously proliferation and lined up in continuous layers. In addition, the urothelial cells on the capsular stents with an embedding period of 2 or 3 weeks showed higher proliferative viability compared with the cells on the stents with an embedding time of 1 week (P < 0.05). The results of the study indicated that the prefabricated capsular stents could serve as alternative cell carriers for tissue engineered ureters, especially with embedding time from 2 to 3 weeks.
Yongde Xu; Weijun Fu; Gang Li; Jianguo Shi; Haisong Tan; Kun Hu; Fuzhai Cui; Qiuxia Lin; Xu Zhang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-2
Journal Detail:
Title:  Journal of materials science. Materials in medicine     Volume:  -     ISSN:  1573-4838     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9013087     Medline TA:  J Mater Sci Mater Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Urology, PLA General Hospital, Military Postgraduate Medical College, No.28 Fuxing Road, Haidian District, Beijing, 100853, China.
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