Document Detail


Autologous buccal keratinocyte implantation for the prevention of stenosis after EMR of the esophagus.
MedLine Citation:
PMID:  17591493     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: EMR is a minimally invasive and well-accepted therapy for early esophageal cancer. However, extensive or circumferential EMR induces stricture formation. Cultured skin is now clinically applicable by using the technology of regenerative medicine. Esophageal mucosa also consists of keratinocytes, the same as with skin. We, therefore, hypothesized that, by applying the technology of regenerative medicine, the stricture occurring in the esophagus after EMR on broad lesions could be prevented. OBJECTIVE: The aim of this study was to evaluate, in a swine model, the effect of autologous keratinocyte implantation at the site after EMR to prevent stricture. DESIGN: With the pig under general anesthesia, EMR was carried out by using the cap technique. Two separate areas were resected by EMR. One area was left as a control. For the other area, autologous buccal keratinocytes were injected endoscopically. MAIN OUTCOME MEASUREMENTS: The outcome, after 2 weeks, was evaluated by endoscopy, macroscopy, and histology. RESULTS: At 2 weeks after EMR, scar formation and stricture were observed in the control lesion. However, in the keratinocyte implanted lesion, the lesion was covered with an epithelium and the luminal surface of the lesion was flat, without ulceration. CONCLUSIONS: These results showed the feasibility of performing autologous keratinocyte implantation after EMR and the effect for early reepithelialization.
Authors:
Tadashi Sakurai; Shukichi Miyazaki; Go Miyata; Susumu Satomi; Yoshio Hori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gastrointestinal endoscopy     Volume:  66     ISSN:  0016-5107     ISO Abbreviation:  Gastrointest. Endosc.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-26     Completed Date:  2007-10-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0010505     Medline TA:  Gastrointest Endosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  167-73     Citation Subset:  IM    
Affiliation:
Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Esophageal Stenosis / etiology*,  pathology,  prevention & control*
Esophagoscopy / adverse effects*
Keratinocytes / transplantation*
Mouth Mucosa / cytology*
Mucous Membrane / surgery
Swine
Tissue Adhesions / etiology,  pathology,  prevention & control
Transplantation, Autologous
Wound Healing

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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