Document Detail

Autologous bone-marrow mononuclear cell implantation improves endothelium-dependent vasodilation in patients with limb ischemia.
MedLine Citation:
PMID:  15007007     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Patients with limb ischemia were associated with endothelial dysfunction. The purpose of this study was to determine whether autologous bone-marrow mononuclear cell (BM-MNC) implantation improves endothelial dysfunction in patients with limb ischemia. METHODS AND RESULTS: We evaluated the leg blood flow (LBF) response to acetylcholine (ACh), an endothelium-dependent vasodilator, and sodium nitroprusside (SNP), an endothelium-independent vasodilator, before and after BM-MNC implantation in 7 patients with limb ischemia. LBF was measured with a mercury-filled Silastic strain-gauge plethysmograph. The number of BM-MNCs implanted into ischemic limbs was 1.6x10(9)+/-0.3x10(9). The number of CD34+ cells included in the implanted BM-MNCs was 3.8x10(7)+/-1.6x10(7). BM-MNC implantation improved the ankle-brachial pressure index (0.33+/-0.21 to 0.39+/-0.17, P=0.06), transcutaneous oxygen pressure (28.4+/-11.5 to 36.6+/-5.2 mm Hg, P=0.03), and pain-free walking time (0.8+/-0.6 to 2.9+/-2.2 minutes, P=0.02). After BM-MNC implantation, LBF response to ACh was enhanced (19.3+/-6.8 versus 29.6+/-7.1 mL/min per 100 mL; P=0.002). The vasodilatory effect of SNP was similar before and after BM-MNC implantation. CONCLUSIONS: These findings suggest that BM-MNC implantation augments endothelium-dependent vasodilation in patients with limb ischemia.
Yukihito Higashi; Masashi Kimura; Keiko Hara; Kensuke Noma; Daisuke Jitsuiki; Keigo Nakagawa; Tetsuya Oshima; Kazuaki Chayama; Taijiro Sueda; Chikara Goto; Hiroaki Matsubara; Toyoaki Murohara; Masao Yoshizumi
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Publication Detail:
Type:  Clinical Trial; Clinical Trial, Phase I; Journal Article     Date:  2004-03-08
Journal Detail:
Title:  Circulation     Volume:  109     ISSN:  1524-4539     ISO Abbreviation:  Circulation     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-16     Completed Date:  2004-06-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1215-8     Citation Subset:  AIM; IM    
Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
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MeSH Terms
Acetylcholine / diagnostic use,  pharmacology
Bone Marrow Cells / physiology*
Bone Marrow Transplantation*
Collateral Circulation*
Endothelium, Vascular / drug effects*,  physiopathology
Ischemia / etiology,  physiopathology,  therapy*
Leg / blood supply*
Middle Aged
Nitroprusside / diagnostic use,  pharmacology
Peripheral Vascular Diseases / complications*
Treatment Outcome
Vasodilator Agents / diagnostic use,  pharmacology
Reg. No./Substance:
0/Vasodilator Agents; 15078-28-1/Nitroprusside; 51-84-3/Acetylcholine

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