| Autologous blood vessels engineered from peripheral blood sample. | |
| | |
MedLine Citation:
|
PMID: 17070080 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Although many efforts have been made to generate small-diameter (< or =5mm) vascular grafts by means of tissue engineering, improvement in patency and functionality still remains a great challenge. It is our hypothesis that to achieve long-term functionality and patency, not only the complete lining with endothelial cells but also full biocompatibility is essential. DESIGN: The aim was the development of a conduit from a scaffold and endothelial progenitor cells (EPC) separated from peripheral blood of a single donor. MATERIALS AND METHODS: EPC and a fibrin preparation were separated from porcine peripheral blood. Fibrin segments were generated seeded with EPC and were perfused in a bioreactor in vitro. RESULTS: From 100ml blood 12-15 cm long fibrin tubes were successfully generated lined with endothelial-like cells. Seeded tubes showed a remarkable elasticity and burst strength up to 90 mm mercury. CONCLUSIONS: Stable fibrin tubes were successfully generated completely lined with an endothelium-like monolayer from fibrin and EPC, both isolated from the same volume of blood. Although their stability is not those needed for arterial grafting, our results raise the hope, that with distinct improvements in future studies functional autologous vascular grafts could be engineered from the patient's own blood. |
| | |
Authors:
|
T Aper; A Schmidt; M Duchrow; H-P Bruch |
Publication Detail:
|
Type: Journal Article Date: 2006-10-25 |
Journal Detail:
|
Title: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery Volume: 33 ISSN: 1078-5884 ISO Abbreviation: Eur J Vasc Endovasc Surg Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2006-12-12 Completed Date: 2007-02-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9512728 Medline TA: Eur J Vasc Endovasc Surg Country: England |
Other Details:
|
Languages: eng Pagination: 33-9 Citation Subset: IM |
Affiliation:
|
Department of General and Vascular Surgery, Klinikum Hannover Oststadt - Heidehaus Hannover, Germany. thomas.aper@t-online.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biocompatible Materials / chemistry Blood Vessel Prosthesis* Blood Vessels / cytology* Cell Differentiation Cell Proliferation Cell Separation / methods Cells, Cultured Elasticity Endothelial Cells / cytology* Fibrin / chemistry Immunohistochemistry Microscopy, Electron, Scanning Stem Cells / cytology* Stress, Mechanical Swine Tensile Strength Tissue Engineering / methods* Transplantation, Autologous |
| Chemical | |
Reg. No./Substance:
|
0/Biocompatible Materials; 9001-31-4/Fibrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Organising a nurse-driven PAD rehabilitation clinic within the vascular surgical department: what is...
Next Document: Anatomical variations in the cephalic and omobrachial veins in the dog.