| Autologous blood pleurodesis in rats to elucidate the amounts of blood required for reliable and reproducible results. | |
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MedLine Citation:
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PMID: 19524261 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Pleurodesis is used in the treatment of spontaneous pneumothorax or refractory pleural effusions of different etiologies. Several agents have been employed, but many questions remain unanswered about their effectiveness and toxicity. Use of autologous blood pleurodesis in clinical practice has been described in the literature without any clear consensus regarding its efficacy. Experimental studies using this technique are limited to a single study in rabbits. We performed a prospective, randomized, observer-blinded, controlled study to evaluate the safety and efficacy of increasing doses of autologous blood pleurodesis in a novel rat model. MATERIALS AND METHODS: Twenty-eight albino Wistar rats were divided into four groups. Groups 1, 2, and 3 were the study groups and group 4 was the control group, with seven animals in each group. Groups 1, 2, and 3 were given autologous blood, 1 mL/kg, 2 mL/kg, 3 mL/kg, respectively, and group 4 (control) was given only 2 mL/kg saline intrapleurally. The rats were sacrificed on postoperative day 30. The surfaces were graded by macroscopic (visible adhesion formation) and microscopic (inflammation and fibrosis) examination. RESULTS: Macroscopically, group 2 and group 3 developed significantly more adhesions; 3 mL/kg autologous blood produced the most significant pleurodesis with generalized adhesions seen between visceral, parietal, and mediastinal pleura. Microscopic examination showed that all study groups developed an inflammatory response at the site of blood injection. There were no pathologic changes in ipsilateral and contralateral lung parenchyma. CONCLUSIONS: Autologous blood at doses 2-3 mL/kg were shown to be effective to produce adhesions in 30 d, and the results were highly reproducible in all rats. We propose that the occasional negative results obtained in humans may be related to an insufficient amount of injected blood, as observed in our rat model. |
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Authors:
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Berkant Ozpolat; Serkal Gazyagci; Alper G?z?b?y?k; Sebnem Ayva; Cansel Atinkaya |
Publication Detail:
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Type: Journal Article Date: 2009-02-23 |
Journal Detail:
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Title: The Journal of surgical research Volume: 161 ISSN: 1095-8673 ISO Abbreviation: J. Surg. Res. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-14 Completed Date: 2010-06-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: 228-32 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Thoracic Surgery, Kirikkale University, School of Medicine, Kirikkale, Turkey. berkantozpolat@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Basement Membrane / pathology, transplantation Catheterization / methods Collagen / metabolism Cystectomy / methods* Dogs Epithelial Cells / physiology Extracellular Matrix / physiology Female Intestinal Mucosa / transplantation Membrane Proteins / metabolism Mice Models, Animal Pleurodesis / methods* Rabbits Rats Swine Tissue Scaffolds* Transplantation, Heterologous Urinary Bladder / pathology, surgery* |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Membrane Proteins; 0/uroplakin II; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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