Document Detail

Autofluorescence of fungi: an aid to detection in tissue sections.
MedLine Citation:
PMID:  6340464     Owner:  NLM     Status:  MEDLINE    
Many pathogenic fungi have been found to autofluoresce in routine hematoxylin and eosin sections when exposed to ultraviolet light. These fungi include Blastomyces, Cryptococcus, Candida, Aspergillus, Coccidioides, and occasionally, Histoplasma. No autofluorescence was observed with Mucor. Examinations of other inflammatory processes, including those producing a granulomatous response, have not revealed any false-positive results. The advantages of fluorescent microscopy for fungal screening include: (1) no special staining procedures required; (2) no time delay, as involved with special stains; (3) the ability to scan sections at a relatively low power; and (4) the ability to tentatively identify the fungus. Disadvantages may include: (1) lack of a fluorescent microscope, and (2) a possible aging process whereby autofluorescence is lost in older cases.
J L Mann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  79     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  1983 May 
Date Detail:
Created Date:  1983-05-27     Completed Date:  1983-05-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  587-90     Citation Subset:  AIM; IM    
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MeSH Terms
Aspergillus / isolation & purification
Blastomyces / isolation & purification
Candida / isolation & purification
Coccidioides / isolation & purification
Cryptococcus / isolation & purification
False Positive Reactions
Fungi / isolation & purification*
Histological Techniques
Histoplasma / isolation & purification
Microscopy, Fluorescence
Mycoses / microbiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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