Document Detail

Autocrine stimulation of Nb2 cell proliferation by secreted, but not intracellular, prolactin.
MedLine Citation:
PMID:  2850497     Owner:  NLM     Status:  MEDLINE    
We have introduced expression constructs for mouse PRL (mPRL) or a nonsecreted form of mPRL into the PRL-responsive Nb2 rat lymphoma cell line. Cell lines resulting from transfection of Nb2 cells with the wild type mPRL construct synthesize and secrete mPRL. These cells are able to grow independently of added lactogens, and conditioned media and cell extracts from these cultures stimulate the growth of Nb2 cells. In contrast, cells synthesizing the nonsecreted mPRL do not proliferate in the absence of added lactogenic hormones, and conditioned media from these cell cultures do not have PRL-like activity in the Nb2 cell growth assay. PRL protein is detected in these nonsecreting cell lines; however, extracts from these lines are generally unable to stimulate Nb2 cell proliferation. These results indicate that cells can respond in an autocrine fashion to PRL, but that an intracellular form of PRL is unable to activate Nb2 cell growth.
J A Davis; D I Linzer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  2     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1989-02-21     Completed Date:  1989-02-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  740-6     Citation Subset:  IM    
Department of Biochemistry, Molecular Biology, and Cell Biology Northwestern University, Evanston, Illinois 60208.
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MeSH Terms
Cell Division
Cell Line
Cloning, Molecular
DNA / genetics
DNA Restriction Enzymes
Prolactin / genetics,  physiology*,  secretion
Protein Biosynthesis
Transcription, Genetic
Grant Support
Reg. No./Substance:
9002-62-4/Prolactin; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

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