| Autocrine growth factor in defined serum-free medium of human salivary gland adenocarcinoma cell line HSG. | |
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MedLine Citation:
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PMID: 2788497 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human salivary gland adenocarcinoma cell line HSG secretes an epidermal growth factor (EGF)-like molecule and contains EGF receptors. Growth of HSG cells is inhibited by glucocorticoid. We have identified that the growth inhibition by glucocorticoid is induced by the reduced secretion of the EGF-like molecule and that addition of anti-human EGF antibody to the culture specifically inhibits the growth of HSG cells, suggesting that autocrine secretion is involved in the growth of HSG cells. To prove that autocrine secretion functions in glucocorticoid-regulated growth of the HSG cell line, we purified the EGF-like molecule from serum-free, defined medium conditioned by the HSG cells and examined the growth-stimulatory effect of the purified molecule. The cultivation of HSG cells in serum-free defined medium, which contains insulin (10 micrograms/ml) and transferrin (10 micrograms/ml) only as proteinaceous components, resulted in establishment of a new cell line (HSG-SF) which had different morphological features from the parental HSG cell line. HSG-SF cells were found to have basically the same responsiveness to glucocorticoid as parental HSG cells. Parental HSG cells secreted high molecular weight EGF-like molecules (Mr 46,000 and 57,000), which were recognized by specific antibody to low molecular weight human EGF (Mr 6,201). From conditioned, serum-free medium of HSG-SF cells, an EGF-like molecule (Mr 46,000) was purified by using an anti-human EGF antibody-coupled Sepharose CL-4B column. This EGF-like molecule induced a maximal increase (36%) in incorporation of [3H]thymidine into DNA of parental HSG cells as well as low molecular weight human EGF. These observations demonstrate that growth of the HSG cell line is regulated by autocrine secretion. |
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Authors:
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R Kurokawa; S Kyakumoto; M Ota |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer research Volume: 49 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1989-10-03 Completed Date: 1989-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5136-42 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Iwate Medical University School of Dentistry, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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pathology*,
secretion Cell Division / drug effects Cell Line Culture Media DNA Replication / drug effects* Epidermal Growth Factor / analysis, secretion Fibronectins / analysis, secretion Humans Salivary Gland Neoplasms / pathology*, secretion Triamcinolone Acetonide / pharmacology* Tumor Cells, Cultured / cytology, drug effects |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fibronectins; 62229-50-9/Epidermal Growth Factor; 76-25-5/Triamcinolone Acetonide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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