| Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa. | |
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MedLine Citation:
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PMID: 21115724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During early urinary tract infection (UTI) the interplay between invading bacteria and the urothelium elicits a mucosal response aimed at clearing infection. Unfortunately, the resultant inflammation and associated local tissue injury are responsible for patient symptoms. Interleukin-6 (IL-6), a cytokine released during acute UTI, has both pro- and anti-inflammatory effects on other body systems. Within the urothelium, the IL-6 native-tissue origin, the target cell type(s), and ultimate effect of the cytokine on target cells are largely unknown. In the present study we modeled the UTI IL-6 response ex vivo using canine bladder mucosa mounted in Ussing chambers to determine the inflammatory and reparative role of IL-6. We demonstrated that uropathogenic Escherichia coli infection stimulates the synthesis of IL-6 by all urothelial cell layers, with the urothelial cells alone representing the only site of unequivocal IL-6 receptor expression. Autocrine effects of IL-6 were supported by the activation of urothelial STAT3 signaling and SOCS3 expression. Using exogenous IL-6, a microarray approach, and quantitative reverse transcriptase PCR (q-RT-PCR), 5 target genes (tumor necrosis factor alpha, interleukin-1β, matrix metallopeptidase 2, heparan sulfate d-glucosaminyl 3-O-sulfotransferase 3A1, and hyaluronan synthase 2) that have direct or indirect roles in promoting a proinflammatory state were identified. Two of these genes, heparan sulfate d-glucosaminyl 3-O-sulfotransferase 3A1 and hyaluronan synthase 2, are also potentially important mediators of wound repair via the production of glycosaminoglycan components. These findings suggest that IL-6 secretion during acute UTI may serve a dual biological role by initiating the inflammatory response while also repairing urothelial defenses. |
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Authors:
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Michael W Wood; Edward B Breitschwerdt; Jody L Gookin |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-11-29 |
Journal Detail:
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Title: Infection and immunity Volume: 79 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-24 Completed Date: 2011-03-15 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 708-15 Citation Subset: IM |
Affiliation:
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Center for Comparative Medicine and Translational Research and Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acute-Phase Reaction
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metabolism* Animals Dogs Humans Interleukin-6 / metabolism* Mucous Membrane / metabolism, microbiology Signal Transduction Tissue Culture Techniques Urinary Bladder / metabolism*, microbiology Uropathogenic Escherichia coli Urothelium / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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T32 RR024394/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6 |
| Comments/Corrections | |
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