Document Detail


Autoantibody profiling on high-density protein microarrays for biomarker discovery in the cerebrospinal fluid.
MedLine Citation:
PMID:  18656479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Detection of autoantibodies, which are involved in tissue injury and/or the reporters from the immune system of various pathologic events, has an important potential for diagnosis, prognosis, disease staging and treatment selection. This explains the interest for new proteomics technologies, such as the high-density protein microarray used here, that allow a high-throughput, multiplexed and sensitive detection of specific autoantibodies. So far, most of the research has been performed on blood. In this note, we focus on the cerebrospinal fluid in an attempt to address autoimmune events associated with neurological disorders. Importantly, the cerebrospinal fluid is quite different from the blood in terms of protein composition and concentration. We had therefore to adapt the available blood protocols. We present here the result of our optimization that will be useful to carry out full scale immunological studies of the cerebrospinal fluid using high-density protein microarrays.
Authors:
Stéphane Roche; Yves Dauvilliers; Laurent Tiers; Carine Couderc; Marie-Thérèse Piva; Monique Provansal; Audrey Gabelle; Sylvain Lehmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-24
Journal Detail:
Title:  Journal of immunological methods     Volume:  338     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-12     Completed Date:  2008-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  75-8     Citation Subset:  IM    
Affiliation:
CHU Montpellier, Laboratoire de Biochimie, Hôpital St. Eloi, Montpellier, F-34000, France.
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MeSH Terms
Descriptor/Qualifier:
Autoantibodies / cerebrospinal fluid*
Biological Markers / cerebrospinal fluid*
Humans
Protein Array Analysis / methods*
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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