| Autoantibody production from a thymoma and a follicular dendritic cell sarcoma associated with paraneoplastic pemphigus. | |
| | |
MedLine Citation:
|
PMID: 16120143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Paraneoplastic pemphigus (PNP) is an autoimmune mucocutaneous disease. We previously reported that B cells in a Castleman tumour associated with PNP produced autoantibodies. However, it is uncertain whether the production of autoantibodies from the associated tumour is a common mechanism in PNP. OBJECTIVES: To investigate autoantibody production in a thymoma and a follicular dendritic cell sarcoma that were excised from two patients with PNP. METHODS: Tumour cells were cultured, and their surface markers were identified. Indirect immunofluorescence, immunoblotting and enzyme-linked immunosorbent assay (ELISA) using culture media from the tumours were used to detect PNP autoantibodies. RESULTS: B cells with markers (CD22+, surface membrane IgG+ and surface membrane IgM+) of mature B lymphocytes constituted a proportion of cultured tumour cells in both tumours. Western blot showed that the medium from both the thymoma and the follicular dendritic cell sarcoma cells recognized 190-kDa periplakin and 210-kDa envoplakin bands of human epithelial proteins as well as recombinant linker regions of periplakin, envoplakin, desmoplakin and bullous pemphigoid antigen 1. ELISA was positive for antidesmoglein 3 antibody. CONCLUSIONS: The presence and localization in tumours of B-lymphocyte clones against proteins of the plakin family and desmoglein 3 in skin may not be confined to PNP with Castleman disease, but is possibly a common mechanism in PNP associated with various tumours. |
| | |
Authors:
|
J Wang; D F Bu; T Li; R Zheng; B X Zhang; X X Chen; X J Zhu |
Related Documents
:
|
12173013 - Hypoxia-induced alteration of tracer accumulation in cultured cancer cells and xenograf... 15886013 - Arginine deprivation and metabolomics: important aspects of intermediary metabolism in ... 7379313 - Size reduction of a prolactin secreting adenoma during long-term treatment with the dop... |
Publication Detail:
|
Type: Case Reports; Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
|
Title: The British journal of dermatology Volume: 153 ISSN: 0007-0963 ISO Abbreviation: Br. J. Dermatol. Publication Date: 2005 Sep |
Date Detail:
|
Created Date: 2005-08-25 Completed Date: 2005-11-14 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0004041 Medline TA: Br J Dermatol Country: England |
Other Details:
|
Languages: eng Pagination: 558-64 Citation Subset: IM |
Affiliation:
|
Department of Dermatology, Peking University First Hospital, 8 Xishiku St, Beijing 100034, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Autoantibodies / analysis* B-Lymphocytes / immunology Biological Markers / analysis Blotting, Western Carrier Proteins / immunology Cytoskeletal Proteins / immunology Dendritic Cells, Follicular / immunology Desmoglein 3 Desmogleins Desmoplakins Enzyme-Linked Immunosorbent Assay Female Fluorescent Antibody Technique Fluorescent Antibody Technique, Indirect Humans Immunophenotyping Membrane Proteins / immunology Middle Aged Nerve Tissue Proteins / immunology Paraneoplastic Syndromes / immunology*, pathology Pemphigus / immunology Plakins Protein Precursors / immunology Retroperitoneal Neoplasms / immunology*, pathology Sarcoma / immunology*, pathology Thymoma / immunology*, pathology Thymus Neoplasms / immunology*, pathology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Autoantibodies; 0/Biological Markers; 0/Carrier Proteins; 0/Cytoskeletal Proteins; 0/DSP protein, human; 0/DST protein, human; 0/Desmoglein 3; 0/Desmogleins; 0/Desmoplakins; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Plakins; 0/Ppl protein, mouse; 0/Protein Precursors; 0/envoplakin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Decreased incidence of nonmelanoma skin cancer in patients with type 2 diabetes mellitus using insul...
Next Document: T-cell receptor gamma gene rearrangement by multiplex polymerase chain reaction/heteroduplex analysi...