Document Detail

Autoantibody production from a thymoma and a follicular dendritic cell sarcoma associated with paraneoplastic pemphigus.
MedLine Citation:
PMID:  16120143     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Paraneoplastic pemphigus (PNP) is an autoimmune mucocutaneous disease. We previously reported that B cells in a Castleman tumour associated with PNP produced autoantibodies. However, it is uncertain whether the production of autoantibodies from the associated tumour is a common mechanism in PNP. OBJECTIVES: To investigate autoantibody production in a thymoma and a follicular dendritic cell sarcoma that were excised from two patients with PNP. METHODS: Tumour cells were cultured, and their surface markers were identified. Indirect immunofluorescence, immunoblotting and enzyme-linked immunosorbent assay (ELISA) using culture media from the tumours were used to detect PNP autoantibodies. RESULTS: B cells with markers (CD22+, surface membrane IgG+ and surface membrane IgM+) of mature B lymphocytes constituted a proportion of cultured tumour cells in both tumours. Western blot showed that the medium from both the thymoma and the follicular dendritic cell sarcoma cells recognized 190-kDa periplakin and 210-kDa envoplakin bands of human epithelial proteins as well as recombinant linker regions of periplakin, envoplakin, desmoplakin and bullous pemphigoid antigen 1. ELISA was positive for antidesmoglein 3 antibody. CONCLUSIONS: The presence and localization in tumours of B-lymphocyte clones against proteins of the plakin family and desmoglein 3 in skin may not be confined to PNP with Castleman disease, but is possibly a common mechanism in PNP associated with various tumours.
J Wang; D F Bu; T Li; R Zheng; B X Zhang; X X Chen; X J Zhu
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  The British journal of dermatology     Volume:  153     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-25     Completed Date:  2005-11-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  558-64     Citation Subset:  IM    
Department of Dermatology, Peking University First Hospital, 8 Xishiku St, Beijing 100034, China.
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MeSH Terms
Autoantibodies / analysis*
B-Lymphocytes / immunology
Biological Markers / analysis
Blotting, Western
Carrier Proteins / immunology
Cytoskeletal Proteins / immunology
Dendritic Cells, Follicular / immunology
Desmoglein 3
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Fluorescent Antibody Technique, Indirect
Membrane Proteins / immunology
Middle Aged
Nerve Tissue Proteins / immunology
Paraneoplastic Syndromes / immunology*,  pathology
Pemphigus / immunology
Protein Precursors / immunology
Retroperitoneal Neoplasms / immunology*,  pathology
Sarcoma / immunology*,  pathology
Thymoma / immunology*,  pathology
Thymus Neoplasms / immunology*,  pathology
Tumor Cells, Cultured
Reg. No./Substance:
0/Autoantibodies; 0/Biological Markers; 0/Carrier Proteins; 0/Cytoskeletal Proteins; 0/DSP protein, human; 0/DST protein, human; 0/Desmoglein 3; 0/Desmogleins; 0/Desmoplakins; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Plakins; 0/Ppl protein, mouse; 0/Protein Precursors; 0/envoplakin

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