Document Detail


Autoantibody against survivin in patients with systemic sclerosis.
MedLine Citation:
PMID:  20551096     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
OBJECTIVE: In patients with systemic sclerosis (SSc), to determine concentrations of antibodies against survivin and their clinical association with SSc, and to evaluate serum survivin concentrations.
METHODS: Anti-survivin antibody was examined by ELISA and immunoblotting using human recombinant survivin. Serum survivin levels were assessed by ELISA.
RESULTS: IgG but not IgM anti-survivin antibody levels in patients with SSc were significantly higher than those in healthy controls and patients with systemic lupus erythematosus (SLE). When cutoff values were set as mean + 2 SD of control, IgG anti-survivin antibodies were positive in 41% (25/61) of patients with SSc, while they were detected in only 1 healthy individual (3%, 1/29) and 1 patient with SLE (5%, 1/20). Regarding the clinical correlation, patients with SSc who were positive for IgG anti-survivin antibody exhibited significantly longer disease duration than those who were negative. Immunoblotting analysis confirmed the presence of anti-survivin antibody in sera from patients with SSc. Serum survivin levels in patients with SSc were also significantly higher than in controls and patients with SLE.
CONCLUSION: Our results suggest that autoantibody against survivin is generated in patients with SSc, especially those with long disease duration.
Authors:
Yuta Koike; Eiji Muroi; Ayumi Yoshizaki; Fumihide Ogawa; Koichi Yanaba; Motoi Takenaka; Kazuhiro Shimizu; Shinichi Sato
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Publication Detail:
Type:  Journal Article     Date:  2010-06-15
Journal Detail:
Title:  The Journal of rheumatology     Volume:  37     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1864-70     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
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