| Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma. | |
| | |
MedLine Citation:
|
PMID: 21455340 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
AIM: To evaluate the diagnostic values of serum autoantibodies against matrix metalloproteinase-7 (MMP-7) in patients with esophageal squamous cell carcinoma (ESCC). METHODS: The MMP-7 cDNA was cloned from ESCC tissues, and MMP-7 was expressed and purified from a prokaryotic system. MMP-7 autoantibodies were then measured in sera from 50 patients with primary ESCC and 58 risk-matched controls, using a reverse capture enzyme-linked immunosorbent assay (ELISA) in which autoantibodies to MMP-7 bound to the purified MMP-7 proteins. In addition, MMP-7 autoantibody levels in sera from 38 gastric cancer patients and from control serum samples were also tested. RESULTS: The optimum conditions for recombinant MMP-7 protein expression were determined as 0.04 mmol/L Isopropyl-β-D-Thiogalactopyranoside (IPTG) induction at 37°C for four hours. The levels of serum autoantibodies against MMP-7 were significantly higher in patients with ESCC than in the matched-control samples (OD450 = 1.69 ± 0.08 vs OD450 = 1.55 ± 0.10, P < 0.001). The area under the receiver operating characteristic (ROC) curve was 0.87. The sensitivity and specificity for detection of ESCC were 78.0% and 81.0%, respectively, when the OD450 value was greater than 1.65. Although the levels of autoantibodies against MMP-7 were also significantly higher in patients with gastric cancer compared to control samples (OD450 = 1.62 ± 0.06 vs OD450 = 1.55 ± 0.10, P < 0.001), the diagnostic accuracy was less significant than in ESCC patients. The area of ROC curve was 0.75, whereas the sensitivity and specificity were 60.5% and 71.7%, respectively, when the cut-off value of OD450 was set at 1.60. CONCLUSION: Serum autoantibody levels of MMP-7 may be a good diagnostic biomarker for esophageal squamous cell carcinoma. |
| | |
Authors:
|
Jing-Hua Zhou; Bin Zhang; Kemp H Kernstine; Li Zhong |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: World journal of gastroenterology : WJG Volume: 17 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-04-01 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
|
Languages: eng Pagination: 1373-8 Citation Subset: IM |
Affiliation:
|
Jing-Hua Zhou, Bin Zhang, Li Zhong, Department of Cell Biology, College of Life Sciences, Hebei University, Baoding 071400, Hebei Province, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Efficacy of endoscopic therapy for gastrointestinal bleeding from Dieulafoy's lesion.
Next Document: Metal stenting to resolve post-photodynamic therapy stricture in early esophageal cancer.