| Auto-induction medium containing glyphosate for high-level incorporation of unusual aromatic amino acids into proteins. | |
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MedLine Citation:
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PMID: 20854268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe the use of an auto-induction medium containing N-(phosphono-methyl)glycine (glyphosate) as a means for high-level introduction of nonstandard aromatic amino acids into a protein. We illustrate this approach by preparing maltose binding protein (MBP) wherein all eight tryptophan residues have been replaced with 6-fluorotryptophan at an incorporation level of 99.3%. Such a high level of incorporation is important for spectroscopic investigations, in particular 19F NMR, because each species' differing amino acid sequence potentially yields a different peak pattern that complicates spectral analysis. |
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Authors:
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Muniasamy Neerathilingam; John L Markley |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: BioTechniques Volume: 49 ISSN: 1940-9818 ISO Abbreviation: BioTechniques Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-21 Completed Date: 2011-01-19 Revised Date: 2011-07-20 |
Medline Journal Info:
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Nlm Unique ID: 8306785 Medline TA: Biotechniques Country: England |
Other Details:
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Languages: eng Pagination: 659-61 Citation Subset: IM |
Affiliation:
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Biochemistry Department, University of Wisconsin-Madison, Madison, WI 53706, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology
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methods Escherichia coli / genetics, metabolism* Glycine / analogs & derivatives*, metabolism Herbicides / metabolism* Maltose-Binding Proteins / chemistry, genetics, metabolism* Recombinant Proteins / chemistry, genetics, metabolism Tryptophan / analogs & derivatives*, chemistry, genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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1U54 GM074901/GM/NIGMS NIH HHS; P41 RR002301-15/RR/NCRR NIH HHS; P41 RR002301-15S1/RR/NCRR NIH HHS; P41 RR002301-16/RR/NCRR NIH HHS; P41 RR002301-17/RR/NCRR NIH HHS; P41 RR002301-18/RR/NCRR NIH HHS; P41 RR002301-19/RR/NCRR NIH HHS; P41 RR002301-20/RR/NCRR NIH HHS; P41 RR002301-21A1/RR/NCRR NIH HHS; P41 RR002301-22/RR/NCRR NIH HHS; P41 RR002301-23/RR/NCRR NIH HHS; P41 RR002301-23S1/RR/NCRR NIH HHS; P41 RR002301-24/RR/NCRR NIH HHS; P41 RR002301-25/RR/NCRR NIH HHS; P41 RR002301-25S1/RR/NCRR NIH HHS; P41 RR002301-26/RR/NCRR NIH HHS; P50 GM064598-01/GM/NIGMS NIH HHS; P50 GM064598-01S1/GM/NIGMS NIH HHS; P50 GM064598-02/GM/NIGMS NIH HHS; P50 GM064598-03/GM/NIGMS NIH HHS; P50 GM064598-03S1/GM/NIGMS NIH HHS; P50 GM064598-04/GM/NIGMS NIH HHS; P50 GM064598-04S1/GM/NIGMS NIH HHS; P50 GM64598/GM/NIGMS NIH HHS; RR02301/RR/NCRR NIH HHS; U54 GM074901-01/GM/NIGMS NIH HHS; U54 GM074901-01S1/GM/NIGMS NIH HHS; U54 GM074901-02/GM/NIGMS NIH HHS; U54 GM074901-02S1/GM/NIGMS NIH HHS; U54 GM074901-03/GM/NIGMS NIH HHS; U54 GM074901-03S1/GM/NIGMS NIH HHS; U54 GM074901-04/GM/NIGMS NIH HHS; U54 GM074901-05/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Herbicides; 0/Maltose-Binding Proteins; 0/Recombinant Proteins; 1071-83-6/glyphosate; 343-92-0/6-fluorotryptophan; 56-40-6/Glycine; 73-22-3/Tryptophan |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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