Document Detail

Authentication of coffee by means of PCR-RFLP analysis and lab-on-a-chip capillary electrophoresis.
MedLine Citation:
PMID:  17002409     Owner:  NLM     Status:  MEDLINE    
Coffee is one of the most important world food commodities, commercial trade consisting almost entirely of Arabica and Robusta varieties. The former is considered to be of superior quality and thus attracts a premium price. Methods to differentiate these coffee species could prove to be beneficial for the detection of either deliberate or accidental adulteration. This study describes a molecular genetics approach to differentiate Arabica and Robusta coffee beans. This employs a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism to monitor a single nucleotide polymorphism within the chloroplastic genome. Samples were analyzed with a lab-on-a-chip capillary electrophoresis system. Coffee powder mixtures were analyzed with this technique, displaying a 5% limit of detection. The plastid copy number was found to be relatively constant across a wide range of bean samples, suggesting that this methodology can also be employed for the quantification of any adulteration of Arabica with Robusta beans.
Stelios Spaniolas; Sean T May; Malcolm J Bennett; Gregory A Tucker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-27     Completed Date:  2006-11-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7466-70     Citation Subset:  IM    
Division of Nutritional Sciences, Authentigene Ltd., School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics LE12 5RD, United Kingdom.
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MeSH Terms
Chloroplasts / genetics
Coffee / classification*,  genetics*
DNA, Plant / analysis*
Electrophoresis, Capillary*
Food Contamination / analysis
Polymerase Chain Reaction*
Polymorphism, Restriction Fragment Length*
Polymorphism, Single Nucleotide
Reg. No./Substance:
0/Coffee; 0/DNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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