|Aurora kinase-A deficiency during skin development impairs cell division and stratification.|
|Jump to Full Text|
|PMID: 22832491 Owner: NLM Status: MEDLINE|
|Aurora kinase-A (Aurora-A) promotes timely entry into mitosis, centrosome maturation, and formation of bipolar spindles. To address the role of Aurora-A in skin development and homeostasis, we interbred a floxed Aurora-A (Aurora-A(fl)) mouse with the Cre-deleter strain, K14.Cre. Aurora-A(fl/fl);Krt14.Cre (Aurora-A(-/-)) mice died shortly after birth. These mice had translucent skin, and histological evaluation showed that the dorsal skin was very thin and fragile with frank erosions. Although the expression of the basal layer marker keratin 14 and the differentiation marker keratin 1 was evident in Aurora-A(-/-) epidermis, there was a marked reduction in the number of suprabasal layers and basal keratinocytes. Dye exclusion assays also showed defects in barrier function. Unlike wild-type cells, Aurora-A(-/-) basal progenitors were delayed in forming two layers at embryonic day (E)13.5 when embryonic skin begins to stratify. Increased numbers of mitotic cells, apoptotic bodies, and polyploid keratinocytes were evident in Aurora-A(-/-) epidermis, indicating that a deficiency in Aurora-A promotes aberrant mitosis, mitotic slippage, and cell death. Finally, Aurora-A(-/-) keratinocytes displayed centrosomal abnormalities that included centrosomes located at nonapical sites in basal cells. Thus, the deletion of Aurora-A in the developing epidermis alters centrosome function of basal keratinocytes and markedly impairs their ability to divide and stratify.|
|Enrique C Torchia; Lei Zhang; Aaron J Huebner; Subrata Sen; Dennis R Roop|
|Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-07-26|
|Title: The Journal of investigative dermatology Volume: 133 ISSN: 1523-1747 ISO Abbreviation: J. Invest. Dermatol. Publication Date: 2013 Jan|
|Created Date: 2013-01-09 Completed Date: 2013-04-02 Revised Date: 2013-10-28|
Medline Journal Info:
|Nlm Unique ID: 0426720 Medline TA: J Invest Dermatol Country: United States|
|Languages: eng Pagination: 78-86 Citation Subset: IM|
|Department of Dermatology, University of Colorado, Denver, CO, USA.|
|APA/MLA Format Download EndNote Download BibTex|
Apoptosis / physiology
Cell Differentiation / genetics, physiology
Cell Division / genetics, physiology*
Centrosome / enzymology
Keratin-14 / biosynthesis
Keratinocytes / physiology
Keratins, Hair-Specific / biosynthesis
Protein-Serine-Threonine Kinases / genetics, physiology*
Skin / enzymology*, growth & development*, pathology
Stem Cells / physiology
|P30 AR057212/AR/NIAMS NIH HHS; P30 CA046934/CA/NCI NIH HHS; P50 CA095103/CA/NCI NIH HHS; R01 CA052607/CA/NCI NIH HHS; R01 CA089716/CA/NCI NIH HHS; R01CA52607/CA/NCI NIH HHS; R01CA89716/CA/NCI NIH HHS|
|0/Keratin-14; 0/Keratins, Hair-Specific; 0/Krt1 protein, mouse; 0/Krt1-14 protein, mouse; EC 220.127.116.11/Protein-Serine-Threonine Kinases; EC 18.104.22.168/aurora kinase|
Journal ID (nlm-journal-id): 0426720
Journal ID (pubmed-jr-id): 4839
Journal ID (nlm-ta): J Invest Dermatol
Journal ID (iso-abbrev): J. Invest. Dermatol.
nihms-submitted publication date: Day: 14 Month: 7 Year: 2012
Electronic publication date: Day: 26 Month: 7 Year: 2012
Print publication date: Month: 1 Year: 2013
pmc-release publication date: Day: 01 Month: 7 Year: 2013
Volume: 133 Issue: 1
First Page: 78 Last Page: 86
PubMed Id: 22832491
|Aurora Kinase A deficiency during skin development impairs cell division and stratification|
|Enrique C. Torchia1|
|Aaron J. Huebner1|
|Dennis R. Roop1*|
1Dept. of Dermatology and Center for Regenerative Medicine and Stem Cell Biology, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO
2Dept. of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston, TX
|*Corresponding Author Mailing address: Department of Dermatology, University of Colorado Anschutz Medical Campus, P.O Box 6511, Aurora, CO, 80045, Telephone: (303)724-3050, Fax: (303)724-3051, Dennis.Roop@UCDenver.edu
Aurora Kinase A (Aurora-A) belongs to a family of conserved mitotic Serine/Threonine kinases that have fundamental roles in the regulation of cellular division. It is found predominantly at the poles of dividing cells, localized to the centrosomes and proximal spindles. Aurora-A is required for the timely entry into mitosis, centrosome maturation, formation of bipolar spindles, and has been implicated in the breakdown of the nuclear envelope and the completion of cytokinesis (Marumoto et al., 2003; Vader and Lens, 2008). Moreover, a role for Aurora-A was recently demonstrated in the control of mitochondrial fission during mitosis (Kashatus et al., 2011). Its protein stability and activity are tightly coupled to the stages of the cell cycle, peaking at the G2/M transition and suppressed at anaphase through protein degradation that is mediated by the Anaphase promoting complex (Honda et al., 2000; Littlepage and Ruderman, 2002). Numerous interacting factors such as AJUBA, BORA, NEDD9, PAK, PLK1 and TPX2 have been identified as important for Aurora-A’s activation and cellular localization (Karthigeyan et al., 2010). Downstream targets of Aurora-A are diverse and growing (Kettenbach et al., 2011; Koch et al., 2011). Of these, Aurora-A can phosphorylate the cell cycle regulator CDC25B and thereby affect the centrosomal redistribution of CDK1–Cyclin B1 complexes to promote mitotic entry (Dutertre et al., 2004). Aurora-A can also activate the tubulin deacetylase, HDAC6, to regulate cilia disassembly (Pugacheva et al., 2007), and phosphorylate NDEL1 to promote neurite extension (Mori et al., 2009). Thus, Aurora-A has cellular functions that go beyond its ability to regulate cell division.
Aurora-A is also a proto-oncogene that is frequently amplified or overexpressed in epithelial cancers including squamous cell carcinomas (SCCs) (Clausen et al., 2006; Torchia et al., 2009). Aurora-A was further identified as a low penetrance cancer susceptibility gene in both humans and mice (Ewart-Toland et al., 2003; Ewart-Toland et al., 2005). Ectopic Aurora-A expression can transform immortalized rodent cell lines in vitro and promote their growth in immunocompromised mice (Bischoff et al., 1998; Zhou et al., 1998). We have previously shown in genetically engineered cancer mouse models that Aurora-A can co-operate with a classical skin chemical carcinogenesis protocol or with the expression of a gain-of-function p53R172H mutant allele to promote the formation of metastasis-prone skin SCCs (Torchia et al., 2012; Torchia et al., 2009). Due to its role in cell division, Aurora-A may be an attractive target for therapeutic intervention in skin cancers. Currently, there are several small molecule inhibitors to Aurora Kinase A and B in clinical trials (Green et al., 2011). However, the long-term off-tumor effects of these inhibitors remain undefined, especially in skin. Inhibition of Aurora-A activity in cultured cells results in mitotic abnormalities, misalignment of chromosomes, and growth suppression (Barr and Gergely, 2007). Recently, three different laboratories have described the global deletion of Aurora-A in mice (Cowley et al., 2009; Lu et al., 2008; Sasai et al., 2008). In these reports, deletion of Aurora-A resulted in embryonic death prior to implantation (Cowley et al., 2009; Lu et al., 2008; Sasai et al., 2008). From these studies, it is clear that Aurora-A is essential for life, but the early lethality of these mice precluded the study of Aurora-A’s function in tissue specific development or homeostasis. In the present study, we examined the consequences of keratinocyte-specific deletion of Aurora-A to determine its role in the formation and maintenance of skin epithelia.
To delete Aurora-A in skin, we interbred floxed Aurora-A mice (Sasai et al., 2008) with a constitutively active Cre recombinase expressed from a truncated Keratin 14 promoter (K14.Cre) (Dassule et al., 2000). This Cre deleter strain begins to express Cre in epithelial tissues at E10.5 and displays Cre activity more uniformly by E12.5 as shown by the activation of the Rosa26 β-galactosidase Cre-reporter (Supplementary Figure 1a). Aurora-Afl/+; K14.Cre mice appeared phenotypically normal. In contrast, Aurora-Afl/fl; K14.Cre mice died shortly after birth, and had obvious skin related abnormalities including fragile, translucent and stretched skin, the failure of eyelids and ears to form normally, fused fingers, and the absence of whisker follicles (Figure 1a). Moreover, toluidine penetration assays in E18.5 embryos revealed defects in skin barrier function, which were more pronounced on the ventral side of the embryos (Figure 1b). Detection of the deleted floxed allele was evident in the skin of both Aurora-Afl/+; K14.Cre and Aurora-Afl/fl; K14.Cre newborn mice (Fig 1c and Supplemental Figure 1b). Deletion of the floxed allele was associated with reduced Aurora-A mRNA levels in whole skin (Supplemental Figure 2a) and the absence of detectable Aurora-A protein in actively dividing keratinocytes of Aurora-Afl/fl; K14.Cre embryos (Supplemental Figure 2b). Collectively, these observations indicate that a defective skin barrier, cause by epidermal Aurora-A deficiency, most likely led to desiccation and perinatal lethality of Aurora-Afl/fl; K14.Cre newborn mice.
Histological evaluation of the skin of Aurora-Afl/fl; K14.Cre mice at E16.5 and older showed a thin epidermis, and the absence of mature hair follicles (Figure 2). We focused on the interfollicular keratinocyte compartment for the remainder of this study. The nuclei of the Aurora-A−/− keratinocytes appeared enlarged with multiple nucleoli. Marker analysis revealed the expression of Krt1 and 10 in the suprabasal compartment, whereas Krt14 and Krt5, and the stress Keratin, Krt6 were evident in the basal layer of Aurora-Afl/fl; K14.Cre dorsal skin (Figure 2). The late differentiation markers, Loricrin and Filaggrin, were also detectable in the outermost layer of Aurora-A−/− epidermis. However, the expression of Filaggrin was markedly reduced or sporadic (Figure 2). A similar pattern of expression was observed for Krt1 on the ventral side of E17.5 Aurora-Afl/fl; K14.Cre embryos, whereas Loricrin and Filaggrin expression was markedly reduced or absent (Supplementary Figure 3). The lack of Loricrin and Filaggrin expression on the ventral side likely contributed to a more severe impairment of barrier function in Aurora-Afl/fl; K14.Cre embryos (see Figure 1b). Both Collagen IV and integrinα6 were found at the epidermal/dermal junction of Aurora-A deficient epidermis, and we observed an expression pattern similar to wildtype (WT) for the adherent junction proteins Zo-1, Desmoglein 1/2 and Desmocolin3 (data not shown). Thus, Aurora-A deficiency did not disrupt the integrity of the basement membrane, or cellular junctions of the epidermis.
The phenotypic presentation and abnormal epidermal architecture of Aurora-Afl/fl; K14.Cre prenatal mice suggested to us that Aurora-A deficiency may impair the formation of the stratified layers of the skin. We therefore examined the embryonic stages between E12–14, when the epithelium stratifies to create the suprabasal compartment (Koster and Roop, 2007). Unlike WT cells, Aurora-A−/− epidermal progenitors were delayed in forming a suprabasal layer in both the ventral and dorsal skin, as evident by histological evaluation and the late appearance of Krt1 expressing cells (Figure 3a). Because suppression of proliferation could account for a delay in the emergence of suprabasal keratinocytes, we examined the S and M phases of the cell cycle in developing skin. At E13.5 similar numbers of keratinocytes in both WT and Aurora-A−/− epidermis were positive for BromodeoxyUridine (BrdU) incorporation indicating that Aurora-A deficiency did not alter the ability of keratinocytes to enter S-phase (Figure 3c). Next, we stained for phospho-Histone H3 to determine the relative number of cells in mitosis (Tapia et al., 2006). As shown in Figure 3b-c for WT epidermis, only a few basal and suprabasal layer cells were positive for phospho-Histone H3, consistent with a short duration spent tranversing M-phase (Dover and Potten, 1988). In contrast, a much higher number of positive cells were found in Aurora-A−/− versus WT skin (a 3 and 6-fold increase in the dorsal and ventral epidermis, respectively). Furthermore, mitotic cells in Aurora-A−/− epidermis lacked a fully formed nuclear envelope as shown by a diffused staining pattern for Lamin A/C, a nuclear membrane structural component (Margalit et al., 2005) (Supplementary Figure 4). As the nuclear envelope is disassembled prior to metaphase in mitosis (Margalit et al., 2005), Aurora-A deficient keratinocytes appear to stall at pro-metaphase of the cell cycle.
Cells that are stalled in mitosis can undergo cell death or escape without completion of cytokinesis by a process that is called mitotic slippage (Rieder and Maiato, 2004). To determine if apoptosis was a consequence of deleting Aurora-A in keratinocytes, we stained Aurora-A−/− epidermis for the apoptotic marker, active Caspase 3. Unlike WT skin, active Caspase 3 was readily detectable in Aurora-A−/− epidermis. Its detection peaked at E13.5, which coincided with the commencement of stratification (Figure 4). Another outcome of a delayed mitosis is the failure to generate two daughter cells, leaving the cell with twice the normal complement of genetic material. To test this possibility, we enumerated centromeres by staining paraffin sections of embryonic skin with a pan-centromere FISH probe. Increased numbers of centromeres were found in Aurora-A−/− epidermis relative to the nuclei found in WT epidermis or in the dermis of Aurora-Aflfl; K14.Cre mice that remained WT for Aurora-A (Figure 5). Moreover, cells in metaphase or anaphase were rarely observed by histology or by staining with Aurora-B, which localizes to the midzone or anaphase bridges during mitosis (Ruchaud et al., 2007) (data not shown). From these analyses, we conclude that Aurora-A−/− keratinocytes gave rise to polyploid cells.
Aurora-A promotes the maturation of centrosomes and formation of bi-polar spindles in preparation for cell division (Vader and Lens, 2008). Therefore, we examined how Aurora-A deficiency affected centrosome function in the developing epidermis. In WT keratinocytes at E16.5 and older, the centrosomes were predominately found apically (Figure 6A, arrows). This orientation was altered in Aurora-A−/− epidermis, with the position of the centrosomes becoming random and located either apically, basolaterally or centrally (Figure 6a, arrows, and see Supplementary Figure 5). As shown in Figure 6b, there were multipolar structures or centrosome clusters in Aurora-A−/− epidermis, reminiscent of spindles anomalies observed in cells with extra centrosomes (Ganem et al., 2009) or those treated with Aurora-A inhibitors, and in Drosophila mutants of Aurora-A (Glover et al., 1995; Manfredi et al., 2007). Since the proper orientation of centrosomes is required for the formation of bi-polar spindles in preparation for metaphase, we next examined the microtubule network in Aurora-A−/− epidermis. In actively dividing WT cells, microtubules are organized between two opposing nucleating poles (i.e., the centrosomes) whose orientation to the basement membrane determines the plane of cell division (Glotzer, 2009). A basal cell that forms a bi-polar spindle perpendicular to the basement membrane will divide asymmetrically and give rise to two cells, a suprabasal and a basal keratinocyte (Figure 6c) (Lechler and Fuchs, 2005; Smart, 1970). In Aurora-A−/− keratinocytes, basal cells with bi-polar spindles were rarely observed at E13.5 or at later embryonic stages. In dividing cells identified by phospho-Histone H3 expression, the microtubule network appeared disorganized (Figure 6c). Taken together, these data indicate that abnormal centrosome function induced by the deletion of Aurora-A leads to a defective cellular division and the mislocation of centrosomes following mitosis of basal cells.
To determine if the effects of Aurora-A deficiency were similar in post-natal skin, Aurora-A floxed mice were crossed with the Tamoxifen-inducible Cre deleter strain, K14.CreER (Vasioukhin et al., 1999). This Cre-fusion shows robust deletion of floxed alleles in keratinocytes both in vitro and in vivo after treatment with Tamoxifen (Tam) (Supplemental Figure 6a). Similar to Aurora-Afl/fl; K14.Cre embryos, Aurora-A was not detectable in dividing keratinocytes present in the skin of Tamoxifen treated Aurora-Afl/fl; K14.CreER mice (Supplemental Figure 6b). Surprisingly, the deletion of Aurora-A in postnatal epidermis resulted in no obvious gross skin abnormalities (e.g., hair loss or skin erosions) 3 months following the induction of Cre activity by Tamoxifen treatment (n=6 and data not shown). We were able to detect cells that were positive for phospho-Histone H3 or active Caspase 3 in Aurorafl/fl; K14.CreER epidermis 10 days after the induction of Cre (Supplementary Figure 7); however, adult epidermis appears to be reasonably tolerant to the deletion of Aurora-A.
In the present study, we utilized a conditional deletion strategy to demonstrate that the expression of Aurora-A is required for normal skin development. The deletion of Aurora-A in embryonic basal keratinocytes resulted in defective centrosome maturation, which markedly impaired the ability of these cells to divide and stratify. Consequently, the initiation of stratification and differentiation was delayed in the developing Aurora-A−/− epidermis, resulting in impaired barrier function and perinatal death. Previous studies have shown that barrier formation begins on the dorsal epidermis and progresses laterally starting at E16 (Hardman et al., 1998). The ventral epidermis is last to acquire a competent barrier. The incorporation and crosslinking of epidermal differentiation proteins such as Loricrin into the cornified envelope coincides with the formation of barrier function (Simpson et al., 2011). In Aurora-A deficient epidermis, barrier formation is defective, leading to a phenotype that is especially prevalent on ventral skin. Nevertheless, dorsal skin in Aurora-Aflfl; K14.Cre embryos was able to express terminal differentiation proteins and form a partial barrier, whereas the ventral epidermis was not. How dorsal skin is able to form two layers and undergo differentiation in light of an obvious cell cycle defect in basal keratinocytes is unclear. It is possible that incomplete Cre-recombination of both floxed Aurora-A alleles between E12.5–13.5 or residual Aurora-A mRNA allowed enough basal progenitors to undergo at least one round of cellular division. Alternatively, basal delamination of non-mitotic cells may contribute to the formation of the first suprabasal layer in Aurora-Afl/fl; K14.Cre skin (Adams and Watt, 1990; Connelly et al., 2010; Liebig et al., 2009; Magee et al., 1987; Poumay et al., 1994).
The phenotypic consequence of deleting Aurora-A in mouse keratinocytes is reminiscent of the effects of expression of Aurora-A mutants in Drosophila (Berdnik and Knoblich, 2002; Glover et al., 1995). Drosophila mutations in Aurora-A result in lower expression of the mutant protein compared to WT flies. The most penetrant of these mutants induced monopolar spindles in dividing cells, delays in mitosis, and polyploidy (Berdnik and Knoblich, 2002; Glover et al., 1995). Invariably, the failure of centrosomes to separate prior to metaphase can explain the effects of suppressing Aurora-A levels in flies. The deletion of Aurora-A in the developing mouse skin mirrored the most severe of the Drosophila mutants, resulting in centrosomal related defects. Additionally, the centrosomes in Aurora-A−/− basal cells appeared to lose the predominant apical placement seen in WT keratinocytes (Lechler and Fuchs, 2007) and were found distributed more randomly. The position of centrosomes, which determines the axis of cell division during mitosis, is set during metaphase in basal keratinocytes (Adams, 1996; Poulson and Lechler, 2010). In the stages that precede metaphase, centrosomes can be found at angles between parallel to perpendicular (0–90°) relative to the basement membrane (Poulson and Lechler, 2010). The observed locations of the centrosomes in Aurora-A−/− keratinocytes may be a consequence of these cells stalling in pro-metaphase, undergoing mitotic slippage, and possibly re-entering the cell cycle.
Surprisingly, the post-natal deletion of Aurora-A resulted in no gross skin defects, suggesting that inhibition of Aurora-A activity may be well tolerated by adult skin, and represents a clear distinction to the effects observed on the prenatal epidermis as we show in this study, or seen in early stage embryos (Cowley et al., 2009; Lu et al., 2008; Sasai et al., 2008). Our observations are also consistent with recent clinical trials of Aurora-A small molecule inhibitors that have shown increased phosho-Histone H3 staining in the skin, but the absence of reported skin-related toxicities (Chakravarty et al., 2011). The mechanism that allows adult skin to tolerate Aurora-A deletion remains unclear but may depend on the relative higher rate of proliferation observed in embryonic versus adult skin. In this regard, Aurora-A inhibition would be expected to have a more profound effect on skin cancer cells, which have a higher rate of proliferation (Torchia et al., 2009), and be more similar to what we observed in embryonic keratinocytes. More importantly, our data suggest that the use of small molecule inhibitors of Aurora-A may be a safe and viable strategy to treat recurrent or metastasis-prone skin SCCs (Ganem et al., 2009) if topical delivery of inhibitors can be achieved.
In summary, we have characterized the cellular and tissue-specific changes associated with Aurora-A deficiency in the developing epidermis. Aurora-A deficient epidermis may be an ideal model to explore the relationship between the regulation of cell division, migration and differentiation in epidermal homeostasis, and a tool to develop novel approaches to maximize the killing of squamous cell carcinoma cells by targeting mitotic regulators.
The previously described floxed Aurora-A(fl) mice (Sasai et al., 2008), K14.Cre mice (Dassule et al., 2000), or K14.CreER mice (Vasioukhin et al., 1999) were interbred to generate Cre positive and heterozygous or homozygous Aurora-Afl mice. Timed matings were set up at night and the presence of vaginal plugs on the following morning noted as day 0. Embryos were then collected at days corresponding to different mouse developmental stages. The University of Colorado Institutional Animal Care and Use Committee approved animal experiments. Detection of the Cre-recombined floxed Aurora-A allele was performed as previously described (Sasai et al., 2008). For experiments using the K14.CreER strain, mice were topically treated with 125 μg of 4 HydroxyTamoxifen (4HOTam) (Sigma) dissolved in ethanol or injected intraperitoneally for 6 days with Tamoxifen (Sigma) (6 mg/40 g body weight) dissolved in sesame oil.
Dye penetration was tested on E18.5 embryos as previously described (Hardman et al., 1998). Briefly, embryos were dehydrated and re-hydrated through a series of methanol washes and subsequently incubated in Phosphate Buffered Saline solution saturated with Toluidine blue (Sigma). RNA isolation, reversed transcription, and QPCR analysis was performed as previously described (Torchia et al., 2009). Aurora-A mRNA was detected by Taqman assay# Mn01248177_m1 (Life technologies) and normalized to Gapdh (Life technologies) levels.
Mouse tissues were fixed in 10% neutral buffered formalin (Thermo Fisher Scientific, Pittsburgh, PA), and embedded in Optimal Cutting Temperature compound (Sakura Finetek) for frozen sectioning or processed for paraffin sectioning. The cut planes were either sagittal at the midline or transverse at the midbody. For histological evaluation, dewaxed 5 μM sections were stained with hematoxylin and eosin (H&E). Immunostaining was performed as previously described with modifications (Torchia et al., 2009). Briefly, paraffin embedded tissue was dewaxed and antigen retrieval performed using a Tris EDTA ph10 solution containing 0.05% Tween20. Sections were then blocked and incubated with the following primary antibodies: Keratin (Krt) 1, 5, 10, 14, Loricrin and Filaggrin (Roop et al., 1984; Yuspa et al., 1989), Col IV (Abcam), Itα6 (Chemicon), γ-tubulin (Abcam), α-tubulin (Sigma), phospho-Histone H3 (Ser 10) (Cell Signaling), Active Capase 3 (Cell Signaling). Appropriate secondary antibodies labeled with Alexa488 or 594 were applied and sections mounted on Vectashield Mounting Medium containing DAPI (Vector labs). Staining detection was performed on a Nikon 90i microscope (Nikon Corporation) using standard techniques. For BromodeoxyUridine (BrdU) in vivo labeling experiments, mice were injected with BrdU (Sigma) as previously described (Torchia et al., 2007). Tissue was then harvested after 2hr, fixed, embedded in paraffin, and stained as described above using rat anti-BrdU antibody (Abcam). Staining for β-galactosidase (X-gal) activity was performed on 10 μm OCT sections as previously described (Torchia et al., 2007).
FISH Detection and enumeration of mouse centromeres was conducted at the University of Colorado Cancer Center Cytogenetic Core. Briefly, dewaxed sections were hybridized with SpectrumRed labeled Poseidon All Mouse Centromere Probe (KI-30500, Kreatech Diagnostics, Durham, NC). Chromatin was counterstained with DAPI (Vectashield Mounting Medium, Vector Laboratories). Analysis was performed on epifluorescence microscope using single interference filters sets for red (Texas red), blue (DAPI), and triple (blue, red, green) band pass filters. Number of signals was scored in at least 30 nuclei per selected area in each specimen. Images were acquired and merged using CytoVision application (Applied Imaging).
To enumerate the number of % keratinocytes that were positive for BrdU or phospho-Histone H3, three separate embryos of each genotype were analyzed. Tissue sections from dorsal and ventral skins at E13.5 were stained for either BrdU incorporation/Krt14 or phospho-Histone H3/Krt14. The number of BrdU or phospho-Histone H3 positive cells were scored at 200x magnification after counting on average 294 and 575 Krt14 positive keratinocytes/embryo, respectively. Similar analysis was performed for active Caspase 3/Krt 14 stained tissue sections with an average of 587 Krt 14 positive cells/embryo counted. Dorsal skin was analyzed in two separate embryos for each embryonic timepoint. Statistical tests shown in Figure 5c (phosho-Histone H3) were performed using GraphPad Prism v5.
FN2CONFLICT OF INTEREST
The authors declare no conflict of interest.
This work was supported by grants from NIH: R01CA52607 (DRR) and RO1CA89716 (SS). We thank Dr. Leila Garcia and the University of Colorado Cancer Center Cytogenetics Core for performing the FISH experiments (P30 CA046934), David Sheneman for technical assistance, and Miss Irene Choi for her assistant in the preparation of this manuscript. We also acknowledge support from the University of Colorado Skin Disease Research Center Morphology and Phenotyping Core facility (P30 AR057212).
|Adams JC,Watt FM. Year: 1990Changes in keratinocyte adhesion during terminal differentiation: reduction in fibronectin binding precedes alpha 5 beta 1 integrin loss from the cell surfaceCell63425352145076|
|Adams RJ. Year: 1996Metaphase spindles rotate in the neuroepithelium of rat cerebral cortexJ Neurosci16761088922417|
|Barr AR,Gergely F. Year: 2007Aurora-A: the maker and breaker of spindle polesJ Cell Sci12029879617715155|
|Berdnik D,Knoblich JA. Year: 2002Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosisCurrent biology: CB12640711967150|
|Bischoff JR,Anderson L,Zhu Y,Mossie K,Ng L,Souza B,et al. Year: 1998A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancersEMBO J173052659606188|
|Chakravarty A,Shinde V,Tabernero J,Cervantes A,Cohen RB,Dees EC,et al. Year: 2011Phase I assessment of new mechanism-based pharmacodynamic biomarkers for MLN8054, a small-molecule inhibitor of Aurora A kinaseCancer Res716758521148750|
|Clausen OP,Aass HC,Beigi M,Purdie KJ,Proby CM,Brown VL,et al. Year: 2006Are keratoacanthomas variants of squamous cell carcinomas? A comparison of chromosomal aberrations by comparative genomic hybridizationJ Invest Dermatol12623081516728973|
|Connelly JT,Gautrot JE,Trappmann B,Tan DW,Donati G,Huck WT,et al. Year: 2010Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisionsNat Cell Biol12711820581838|
|Cowley DO,Rivera-Perez JA,Schliekelman M,He YJ,Oliver TG,Lu L,et al. Year: 2009Aurora-A kinase is essential for bipolar spindle formation and early developmentMol Cell Biol2910597119075002|
|Dassule HR,Lewis P,Bei M,Maas R,McMahon AP. Year: 2000Sonic hedgehog regulates growth and morphogenesis of the toothDevelopment12747758511044393|
|Dover R,Potten CS. Year: 1988Heterogeneity and cell cycle analyses from time-lapse studies of human keratinocytes in vitroJ Cell Sci89 Pt 3359642461952|
|Dutertre S,Cazales M,Quaranta M,Froment C,Trabut V,Dozier C,et al. Year: 2004Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transitionJ Cell Sci11725233115128871|
|Ewart-Toland A,Briassouli P,de Koning JP,Mao JH,Yuan J,Chan F,et al. Year: 2003Identification of Stk6/STK15 as a candidate low-penetrance tumor-susceptibility gene in mouse and humanNat Genet344031212881723|
|Ewart-Toland A,Dai Q,Gao YT,Nagase H,Dunlop MG,Farrington SM,et al. Year: 2005Aurora-A/STK15 T+91A is a general low penetrance cancer susceptibility gene: a meta-analysis of multiple cancer typesCarcinogenesis2613687315802297|
|Ganem NJ,Godinho SA,Pellman D. Year: 2009A mechanism linking extra centrosomes to chromosomal instabilityNature4602788219506557|
|Glotzer M. Year: 2009The 3Ms of central spindle assembly: microtubules, motors and MAPsNat Rev Mol Cell Biol1092019197328|
|Glover DM,Leibowitz MH,McLean DA,Parry H. Year: 1995Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindlesCell81951057720077|
|Green MR,Woolery JE,Mahadevan D. Year: 2011Update on Aurora Kinase Targeted Therapeutics in OncologyExpert Opin Drug Discov629130721556291|
|Hardman MJ,Sisi P,Banbury DN,Byrne C. Year: 1998Patterned acquisition of skin barrier function during developmentDevelopment1251541529502735|
|Honda K,Mihara H,Kato Y,Yamaguchi A,Tanaka H,Yasuda H,et al. Year: 2000Degradation of human Aurora2 protein kinase by the anaphase-promoting complex-ubiquitin-proteasome pathwayOncogene192812910851084|
|Karthigeyan D,Prasad SB,Shandilya J,et al. Year: 2011Biology of Aurora A kinase: implications in cancer manifestation and therapyMed Res Rev31757793|
|Kashatus DF,Lim KH,Brady DC,Pershing NL,Cox AD,Counter CM. Year: 2011RALA and RALBP1 regulate mitochondrial fission at mitosisNat Cell Biol1311081521822277|
|Kettenbach AN,Schweppe DK,Faherty BK,Pechenick D,Pletnev AA,Gerber SA. Year: 2011Quantitative phosphoproteomics identifies substrates and functional modules of Aurora and Polo-like kinase activities in mitotic cellsSci Signal4rs521712546|
|Koch A,Krug K,Pengelley S,Macek B,Hauf S. Year: 2011Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeastSci Signal4rs621712547|
|Koster MI,Roop DR. Year: 2007Mechanisms regulating epithelial stratificationAnnu Rev Cell Dev Biol239311317489688|
|Lechler T,Fuchs E. Year: 2005Asymmetric cell divisions promote stratification and differentiation of mammalian skinNature4372758016094321|
|Lechler T,Fuchs E. Year: 2007Desmoplakin: an unexpected regulator of microtubule organization in the epidermisJ Cell Biol1761475417227889|
|Liebig T,Erasmus J,Kalaji R,Davies D,Loirand G,Ridley A,et al. Year: 2009RhoE Is required for keratinocyte differentiation and stratificationMol Biol Cell204526318923151|
|Littlepage LE,Ruderman JV. Year: 2002Identification of a new APC/C recognition domain, the A box, which is required for the Cdh1-dependent destruction of the kinase Aurora-A during mitotic exitGenes Dev1622748512208850|
|Lu LY,Wood JL,Ye L,Minter-Dykhouse K,Saunders TL,Yu X,et al. Year: 2008Aurora A is essential for early embryonic development and tumor suppressionJ Biol Chem283317859018801727|
|Magee AI,Lytton NA,Watt FM. Year: 1987Calcium-induced changes in cytoskeleton and motility of cultured human keratinocytesExperimental cell research17243532443374|
|Manfredi MG,Ecsedy JA,Meetze KA,Balani SK,Burenkova O,Chen W,et al. Year: 2007Antitumor activity of MLN8054, an orally active small-molecule inhibitor of Aurora A kinaseProc Natl Acad Sci U S A10441061117360485|
|Margalit A,Vlcek S,Gruenbaum Y,Foisner R. Year: 2005Breaking and making of the nuclear envelopeJournal of cellular biochemistry954546515832341|
|Marumoto T,Honda S,Hara T,Nitta M,Hirota T,Kohmura E,et al. Year: 2003Aurora-A kinase maintains the fidelity of early and late mitotic events in HeLa cellsJ Biol Chem278517869514523000|
|Mori D,Yamada M,Mimori-Kiyosue Y,Shirai Y,Suzuki A,Ohno S,et al. Year: 2009An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite elongation by modulation of microtubule dynamicsNat Cell Biol1110576819668197|
|Poulson ND,Lechler T. Year: 2010Robust control of mitotic spindle orientation in the developing epidermisJ Cell Biol1919152221098114|
|Poumay Y,Roland IH,Leclercq-Smekens M,Leloup R. Year: 1994Basal detachment of the epidermis using dispase: tissue spatial organization and fate of integrin alpha 6 beta 4 and hemidesmosomesJ Invest Dermatol10211178288902|
|Pugacheva EN,Jablonski SA,Hartman TR,Henske EP,Golemis EA. Year: 2007HEF1-dependent Aurora A activation induces disassembly of the primary ciliumCell12913516317604723|
|Rieder CL,Maiato H. Year: 2004Stuck in division or passing through: what happens when cells cannot satisfy the spindle assembly checkpointDev Cell76375115525526|
|Roop DR,Cheng CK,Titterington L,Meyers CA,Stanley JR,Steinert PM,et al. Year: 1984Synthetic peptides corresponding to keratin subunits elicit highly specific antibodiesJ Biol Chem2598037406203901|
|Ruchaud S,Carmena M,Earnshaw WC. Year: 2007Chromosomal passengers: conducting cell divisionNat Rev Mol Cell Biol879881217848966|
|Sasai K,Parant JM,Brandt ME,Carter J,Adams HP,Stass SA,et al. Year: 2008Targeted disruption of Aurora A causes abnormal mitotic spindle assembly, chromosome misalignment and embryonic lethalityOncogene274122718345035|
|Simpson CL,Patel DM,Green KJ. Year: 2011Deconstructing the skin: cytoarchitectural determinants of epidermal morphogenesisNat Rev Mol Cell Biol125658021860392|
|Smart IH. Year: 1970Variation in the plane of cell cleavage during the process of stratification in the mouse epidermisBr J Dermatol82276825441760|
|Tapia C,Kutzner H,Mentzel T,Savic S,Baumhoer D,Glatz K. Year: 2006Two mitosis-specific antibodies, MPM-2 and phospho-histone H3 (Ser28), allow rapid and precise determination of mitotic activityThe American journal of surgical pathology3083916330946|
|Torchia EC,Boyd K,Rehg JE,Qu C,Baker SJ. Year: 2007EWS/FLI-1 induces rapid onset of myeloid/erythroid leukemia in miceMol Cell Biol2779183417875932|
|Torchia EC,Caulin C,Acin S,Terzian T,Kubick BJ,Box NF,et al. Year: 2012Myc, Aurora Kinase A, and mutant p53(R172H) co-operate in a mouse model of metastatic skin carcinomaOncogene3126809021963848|
|Torchia EC,Chen Y,Sheng H,Katayama H,Fitzpatrick J,Brinkley WR,et al. Year: 2009A genetic variant of Aurora kinase A promotes genomic instability leading to highly malignant skin tumorsCancer Res6972071519738056|
|Vader G,Lens SM. Year: 2008The Aurora kinase family in cell division and cancerBiochim Biophys Acta1786607218662747|
|Vasioukhin V,Degenstein L,Wise B,Fuchs E. Year: 1999The magical touch: genome targeting in epidermal stem cells induced by tamoxifen application to mouse skinProc Natl Acad Sci U S A968551610411913|
|Yuspa SH,Kilkenny AE,Steinert PM,Roop DR. Year: 1989Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in vitroJ Cell Biol1091207172475508|
|Zhou H,Kuang J,Zhong L,Kuo WL,Gray JW,Sahin A,et al. Year: 1998Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformationNat Genet20189939771714|
Keywords: skin development, Aurora Kinase A, centrosomes.
Previous Document: TGF?1 overexpression by keratinocytes alters skin dendritic cell homeostasis and enhances contact hy...
Next Document: Inhibition of CRM1-Mediated Nucleocytoplasmic Transport: Triggering Human Melanoma Cell Apoptosis by...