| Augmented survival of Neisseria gonorrhoeae within biofilms: exposure to atmospheric pressure non-thermal plasmas. | |
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MedLine Citation:
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PMID: 20839022 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Bacteria embedded within biofilms present a challenge to surface decontamination by conventional means. Atmospheric pressure non-thermal plasma processes have emerged as a promising approach to overcoming this problem. We used atmospheric pressure non-equilibrium plasmas (APNPs) to assess planktonic versus biofilm-resident bacterial (Neisseria gonorrhoeae) susceptibility to treatment. The decontamination efficiency of the process was evaluated against bacteria embedded within a biofilm, as well as planktonic cells placed on a glass surface. Bacterial survival was assessed using a combination of colony forming unit (CFU) ability and vital staining with a combination of SYTO9 plus propidium iodide. Both methods revealed an increased resistance of biofilm-resident bacteria compared with planktonic cells, after a 20-min exposure to the APNPs. Transmission electron microscopy revealed disruption and damage to the cell wall, resulting in the release of cytoplasmic compounds, alterations in morphology, and a decrease in cell volume, indicating that APNPs may affect the cell wall. Present results show that biofilm-resident bacteria demonstrate augmented survival when exposed to APNP treatment and therefore that decontamination procedures should take into account this survival when evaluating surface decontamination measures. |
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Authors:
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L Xu; Y Tu; Y Yu; M Tan; J Li; H Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-14 |
Journal Detail:
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Title: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology Volume: 30 ISSN: 1435-4373 ISO Abbreviation: Eur. J. Clin. Microbiol. Infect. Dis. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804297 Medline TA: Eur J Clin Microbiol Infect Dis Country: Germany |
Other Details:
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Languages: eng Pagination: 25-31 Citation Subset: IM |
Affiliation:
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Department of Dermatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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