Document Detail


Augmented survival of Neisseria gonorrhoeae within biofilms: exposure to atmospheric pressure non-thermal plasmas.
MedLine Citation:
PMID:  20839022     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Bacteria embedded within biofilms present a challenge to surface decontamination by conventional means. Atmospheric pressure non-thermal plasma processes have emerged as a promising approach to overcoming this problem. We used atmospheric pressure non-equilibrium plasmas (APNPs) to assess planktonic versus biofilm-resident bacterial (Neisseria gonorrhoeae) susceptibility to treatment. The decontamination efficiency of the process was evaluated against bacteria embedded within a biofilm, as well as planktonic cells placed on a glass surface. Bacterial survival was assessed using a combination of colony forming unit (CFU) ability and vital staining with a combination of SYTO9 plus propidium iodide. Both methods revealed an increased resistance of biofilm-resident bacteria compared with planktonic cells, after a 20-min exposure to the APNPs. Transmission electron microscopy revealed disruption and damage to the cell wall, resulting in the release of cytoplasmic compounds, alterations in morphology, and a decrease in cell volume, indicating that APNPs may affect the cell wall. Present results show that biofilm-resident bacteria demonstrate augmented survival when exposed to APNP treatment and therefore that decontamination procedures should take into account this survival when evaluating surface decontamination measures.
Authors:
L Xu; Y Tu; Y Yu; M Tan; J Li; H Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-14
Journal Detail:
Title:  European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology     Volume:  30     ISSN:  1435-4373     ISO Abbreviation:  Eur. J. Clin. Microbiol. Infect. Dis.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804297     Medline TA:  Eur J Clin Microbiol Infect Dis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
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