Document Detail

Augmented hypothalamic proopiomelanocortin gene expression with pubertal development in the male rat: evidence for an androgen receptor-independent action.
MedLine Citation:
PMID:  1989845     Owner:  NLM     Status:  MEDLINE    
To investigate the mechanism(s) during pubertal development by which androgens alter hypothalamic proopiomelanocortin (POMC) gene expression and beta-endorphin content, we used the technique of in situ hybridization histochemistry and the androgen-insensitive testicular feminized (Tfm) rat. We evaluated POMC mRNA levels in the arcuate nuclei and periarcuate regions of 12 coronal brain slices from prepubertal (age, 30 days) and adult (age, 60 days) normal male and Tfm rats (n = 4 for each group). Hybridizations were performed using an 35S-radiolabeled oligonucleotide probe complementary to a 30-base sequence within POMC mRNA. The tissue sections were sequentially exposed to x-ray film and photographic emulsion with subsequent analysis by both densitometry and computer-assisted grain counting. beta-Endorphin was measured in hypothalamic tissue blocks from similar animals in each of the four experimental groups. The results of densitometry and grain counting were consistent and revealed an increase in POMC mRNA with pubertal development in both the male and Tfm animals. The concentration of hypothalamic beta-endorphin was greater for the adult Tfm animals than for all other groups, which did not differ from each other. These results suggest that androgens may stimulate POMC gene transcription by their action through estrogen receptors after conversion by aromatase. Alternatively, additional pubertal factors may be responsible for act directly through their respective receptors to alter translation, posttranslational processing, or secretion of beta-endorphin, resulting in diminished intracellular hypothalamic peptide concentration.
J R Kerrigan; P M Martha; R J Krieg; T A Queen; P E Monahan; A D Rogol
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  128     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-03-04     Completed Date:  1991-03-04     Revised Date:  2010-04-12    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1029-35     Citation Subset:  AIM; IM    
Department of Pediatrics, University of Virginia Health Sciences Center, Charlottesville 22908.
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MeSH Terms
Body Weight
Gene Expression Regulation*
Gonadal Steroid Hormones / blood
Hypothalamus / metabolism*
Nucleic Acid Hybridization
Osmolar Concentration
Pro-Opiomelanocortin / genetics*
Receptors, Androgen / physiology*
beta-Endorphin / metabolism
Grant Support
Reg. No./Substance:
0/Gonadal Steroid Hormones; 0/Receptors, Androgen; 60617-12-1/beta-Endorphin; 66796-54-1/Pro-Opiomelanocortin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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