Document Detail

Augmented damage of islets by impaired exocrine acinar cells undergoing apoptosis that is possibly converted to necrosis during isolation.
MedLine Citation:
PMID:  21508665     Owner:  NLM     Status:  MEDLINE    
Islet damage attributed to impaired exocrine cells during pancreas preservation and isolation procedure remains elusive, although released exocrine enzymes could directly damage islets. The aim of this study is to investigate the cellular mechanisms associated with exocrine cells and their possible impact on the islet cell survival and function after isolation. Mouse pancreata were stored in cold University of Wisconsin preservation solution for 0, 24 and 48 h and incubated with or without collagenase at 37°C for 15 min. During preservation, the percentage of exocrine cells with necrosis, which means impaired cellular membrane that allows intracellular enzymes to be released, remains low (< 10%) regardless of preservation time; whereas the percentage of exocrine cells with apoptosis, which means impaired nucleus and possible intact cellular membrane, increases over time of preservation. After collagenase-free incubation, however, the percentage of exocrine cells with necrosis became higher in longer preservation time, and more than 60% of the necrotic exocrine cells contained apoptosis as well. Islet cells located in pancreata with intact structure are almost kept away either from necrotic or apoptotic changes even after 48 h preservation followed by collagenase-free incubation. However, when islets are isolated after collagenase-containing incubation, the percentage of islet cells with necrosis increases over time of preservation up to approximately 40%. This study suggests that exocrine cells with necrosis could cause damage of isolated islets when the pancreas is dissociated and that the necrosis in exocrine cells might be induced mainly as the conversion from apoptosis that has already existed during preservation.
Hamed Elgendy; Teru Okitsu; Yasuko Kimura; Xibao Liu; Hanaa Nafady-Hego; Jiro Kurata; Hiroki Teramae; Ashraf Elbahrawy; Shinji Uemoto; Kazuhiko Fukuda
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Publication Detail:
Type:  Journal Article     Date:  2011-05-01
Journal Detail:
Title:  Islets     Volume:  3     ISSN:  1938-2022     ISO Abbreviation:  Islets     Publication Date:    2011 May-Jun
Date Detail:
Created Date:  2011-05-12     Completed Date:  2011-09-27     Revised Date:  2014-10-09    
Medline Journal Info:
Nlm Unique ID:  101495366     Medline TA:  Islets     Country:  United States    
Other Details:
Languages:  eng     Pagination:  102-10     Citation Subset:  IM    
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MeSH Terms
Apoptosis / physiology*
Cell Survival / drug effects,  physiology
Collagenases / metabolism
Diabetes Mellitus, Experimental / metabolism,  pathology,  surgery
In Situ Nick-End Labeling
Islets of Langerhans / cytology*,  pathology
Mice, Inbred C57BL
Organ Preservation Solutions / metabolism*
Specific Pathogen-Free Organisms
Reg. No./Substance:
0/Organ Preservation Solutions; EC 3.4.24.-/Collagenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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