Document Detail


Augmented cardiac cardiotrophin-1 in experimental congestive heart failure.
MedLine Citation:
PMID:  10618298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Cardiotrophin-1 (CT-1) is a potent hypertrophic factor discovered by coupling expression cloning in a mouse embryonic stem cell-based model of cardiogenesis. METHODS AND RESULTS: The present study was designed to investigate the potential activation of atrial and ventricular CT-1 expression in pacing-induced experimental congestive heart failure (CHF) and its relationship to left ventricular hypertrophy by the method of Northern blot analysis and immunohistochemistry. We used a canine model of pacing-induced experimental CHF based on hemodynamic and neurohumoral characteristics that closely mimic human dilated cardiomyopathy. Northern blot analysis demonstrated that CT-1 gene expression was present in normal atrium and ventricle and was increased in CHF hearts. There was a positive correlation between ventricular CT-1 mRNA and left ventricular mass index. Immunohistochemistry revealed positive immunostaining in the atrial and ventricular cardiomyocytes from both normal and CHF hearts. CT-1 immunoreactivity was more intense in the atrium and ventricle from CHF hearts than in normal hearts. CONCLUSIONS: The present study demonstrates that both atrium and ventricle synthesize CT-1 and that cardiac production of CT-1 is augmented in a canine model of experimental CHF. This study also demonstrates that ventricular CT-1 mRNA correlates with left ventricular hypertrophy, suggesting that CT-1 plays an important role in the structural remodeling that characterizes CHF.
Authors:
M Jougasaki; I Tachibana; A Luchner; H Leskinen; M M Redfield; J C Burnett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation     Volume:  101     ISSN:  0009-7322     ISO Abbreviation:  Circulation     Publication Date:    2000 Jan 4-11
Date Detail:
Created Date:  2000-02-24     Completed Date:  2000-02-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14-7     Citation Subset:  AIM; IM    
Affiliation:
Cardiorenal Research Laboratory, Mayo Clinic, Rochester, MN 55905, USA. jougasaki@mayo.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Cardiomyopathy, Dilated / metabolism,  physiopathology
Cytokines / analysis,  genetics*
Disease Models, Animal
Dogs
Gene Expression Regulation*
Heart Failure / metabolism*,  physiopathology*
Heart Ventricles
Hemodynamics
Humans
Hypertrophy, Left Ventricular / metabolism,  physiopathology
Immunohistochemistry
Mice
Myocardium / metabolism*
RNA, Messenger / genetics
Grant Support
ID/Acronym/Agency:
HL-36634/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/RNA, Messenger; 0/cardiotrophin 1

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