| Augmentation of rat skin flap viability by relaxin-expressing adenovirus. | |
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MedLine Citation:
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PMID: 22092841 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Relaxin (RLX) has multiple vascular actions, including vasodilation and angiogenesis, which occur via induction of vascular endothelial growth factor (VEGF) expression. We generated a RLX-expressing (dE1-RGD/lacZ/RLX) adenovirus and investigated whether it enhances skin flap survival. Thirty Sprague-Dawley rats were divided into three groups: RLX-expressing adenovirus group, control virus group, and phosphate-buffered saline (PBS) group. Two days before surgery and immediately after flap elevation, the caudally based flap that was 3 × 9 cm in size was subdermally injected with the dE1-RGD/lacZ/RLX virus (10(7) PFU), dE1-RGD/lacZ virus (10(7) PFU), or PBS. The surviving area of the flap and the amount of blood flow were measured. On postoperative day 10, CD31-positive vessels and VEGF protein expression were examined. We observed a significant increase in the survival area of the flap in the RLX group. Doppler measurement also showed significantly increased blood flow immediately after the operation and on postoperative days 7 and 10. CD31-positive vessels and VEGF protein expression were significantly greater in the RLX group. Thus, administration of RLX-expressing adenovirus into elevated skin flaps increased VEGF expression, the number of capillaries, and blood flow to the flap, thereby improving skin flap survival. |
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Authors:
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Won Jai Lee; Chae-Ok Yun; In Sik Yun; Yong-Oock Kim; Il-Kyu Choi; Tae Jin Yun; Dong Kyun Rah |
Publication Detail:
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Type: Journal Article Date: 2011-10-12 |
Journal Detail:
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Title: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society Volume: 19 ISSN: 1524-475X ISO Abbreviation: Wound Repair Regen Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9310939 Medline TA: Wound Repair Regen Country: United States |
Other Details:
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Languages: eng Pagination: 709-17 Citation Subset: IM |
Copyright Information:
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© 2011 by the Wound Healing Society. |
Affiliation:
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Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University, Seoul, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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