Document Detail


Augmentation of cardiac protein delivery using ultrasound targeted microbubble destruction.
MedLine Citation:
PMID:  15866418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gas-filled microbubbles have become an important tool as ultrasonic contrast agents. We have previously shown that ultrasound-targeted microbubble destruction (UTMD) can direct plasmids to the heart. The aim of this study was to evaluate UTMD for protein delivery. Six different groups of rats received 1 microg of luciferase protein with varying protocols: (1) luciferase-loaded microbubbles and ultrasound; (2) luciferase only; (3) luciferase and ultrasound; (4) luciferase-loaded microbubbles; (5) unloaded microbubbles incubated with luciferase and ultrasound; (6) unloaded microbubbles with ultrasound followed by luciferase. Relative luminescence units per mg protein per s were determined in hearts and control organs. The rats that received ultrasound and luciferase-loaded bubbles showed a six-fold higher cardiac luciferase uptake compared with control groups that did not include bubbles. None of the other groups significantly augmented cardiac luciferase activity. We conclude that ultrasound-targeted microbubble destruction can substantially and noninvasively augment organ-specific delivery of proteins.
Authors:
Raffi Bekeredjian; Shuyuan Chen; Paul A Grayburn; Ralph V Shohet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ultrasound in medicine & biology     Volume:  31     ISSN:  0301-5629     ISO Abbreviation:  Ultrasound Med Biol     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-03     Completed Date:  2005-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410553     Medline TA:  Ultrasound Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  687-91     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, NB 11.200, Dallas, TX 75390-8573, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Contrast Media
Echocardiography
Heart
Luciferases / administration & dosage*
Luminescent Proteins / administration & dosage*
Microbubbles
Rats
Ultrasonics*
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Luminescent Proteins; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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