Document Detail

Augmentation by aphidicolin of 1-beta-D-arabinofuranosylcytosine-induced c-jun and NF-kappa B activation in a human myeloid leukemia cell line: correlation with apoptosis.
MedLine Citation:
PMID:  7564475     Owner:  NLM     Status:  MEDLINE    
1-beta-D-arabinofuranosylcytosine (ara-C) (2 microM) can induce apoptosis in a human myeloid leukemia cell line, U937, after 4 h of incubation. Pretreatment of cells with aphidicolin (2 microM) augments ara-C-induced apoptosis, since it was first observed at 0.4 microM ara-C and became more intense at 2 and 10 microM. Although aphidicolin itself had a marginal effect on c-jun expression, it significantly augmented ara-C induced c-jun upregulation by shortening the lag time and lowering ara-C concentrations necessary for the induction of detectable c-jun transcripts. Aphidicolin and ara-C acted synergistically to increase NF-kappa B DNA binding activity as determined by an electrophoretic mobility shift assay. Expression of c-myc was slightly increased through the DNA degradative phase, and was then downregulated. Thus, the activation of NF-kappa B and c-jun expression seems to be well correlated with the potentiation by aphidicolin of ara-C-induced apoptosis.
K Kuwakado; M Kubota; R Bessho; A Kataoka; I Usami; Y W Lin; A Okuda; Y Wakazono
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Leukemia research     Volume:  19     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-11-22     Completed Date:  1995-11-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  645-50     Citation Subset:  IM    
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
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MeSH Terms
Antimetabolites, Antineoplastic / administration & dosage*
Aphidicolin / administration & dosage*
Apoptosis / drug effects*
Base Sequence
Cytarabine / administration & dosage*
DNA Damage / drug effects
DNA Polymerase II / antagonists & inhibitors*
Drug Synergism
Enzyme Inhibitors / administration & dosage*
Gene Expression Regulation, Neoplastic / drug effects
Genes, jun*
Genes, myc
Molecular Sequence Data
NF-kappa B / genetics*
Oligodeoxyribonucleotides / chemistry
Proto-Oncogene Proteins c-jun / genetics*
RNA, Messenger / genetics
Tumor Cells, Cultured
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/Enzyme Inhibitors; 0/NF-kappa B; 0/Oligodeoxyribonucleotides; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 147-94-4/Cytarabine; 38966-21-1/Aphidicolin; EC 2.7.7.-/DNA Polymerase II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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