Document Detail


Augmentation of therapeutic efficacy in drug-resistant tumor models using ceramide coadministration in temporal-controlled polymer-blend nanoparticle delivery systems.
MedLine Citation:
PMID:  20143195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of multidrug resistance (MDR) is a major hindrance to cancer eradication as it renders tumors unresponsive to most chemotherapeutic treatments and is associated with cancer resurgence. This study describes a novel mechanism to overcome MDR through a polymer-blend nanoparticle platform that delivers a combination therapy of C6-ceramide (CER), a synthetic analog of an endogenously occurring apoptotic modulator, together with the chemotherapeutic drug paclitaxel (PTX), in a single formulation. The PTX/CER combination therapy circumvents another cellular mechanism whereby MDR develops, by lowering the threshold for apoptotic signaling. In vivo studies in a resistant subcutaneous SKOV3 human ovarian and in an orthotopic MCF7 human breast adenocarcinoma xenograft showed that the PTX and CER nanoparticle combination therapy reduced the final tumor volume at least twofold over treatment with the standard PTX therapy alone. The study also revealed that the cotherapy accomplished this enhanced efficacy by generating an enhancement in apoptotic signaling in both tumor types. Additionally, acute evaluation of safety with the combination therapy did not show significant changes in body weight, white blood cell counts, or liver enzyme levels. The temporal-controlled nanoparticle delivery system presented in this study allows for a simultaneous delivery of PTX + CER in breast and ovarian tumor model drug, leading to a modulation of the apoptotic threshold. This strategy has tremendous potential for effective treatment of refractory disease in cancer patients.
Authors:
Lilian E van Vlerken; Zhenfeng Duan; Steven R Little; Michael V Seiden; Mansoor M Amiji
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-02-09
Journal Detail:
Title:  The AAPS journal     Volume:  12     ISSN:  1550-7416     ISO Abbreviation:  AAPS J     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-03-24     Completed Date:  2010-06-15     Revised Date:  2011-07-25    
Medline Journal Info:
Nlm Unique ID:  101223209     Medline TA:  AAPS J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  171-80     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, Massachusetts 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / administration & dosage
Antineoplastic Agents, Phytogenic / administration & dosage
Antineoplastic Combined Chemotherapy Protocols / administration & dosage,  adverse effects,  therapeutic use*
Body Weight / drug effects
Cell Line, Tumor
Ceramides / administration & dosage
Chemistry, Pharmaceutical
Drug Delivery Systems
Drug Resistance, Multiple
Drug Resistance, Neoplasm*
Female
Humans
In Situ Nick-End Labeling
Leukocyte Count
Liver / enzymology
Mice
Mice, Nude
Nanoparticles
P-Glycoprotein / genetics
Paclitaxel / administration & dosage
Polymers
Solubility
Grant Support
ID/Acronym/Agency:
R01-CA119617/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/ABCB1 protein, human; 0/Antineoplastic Agents; 0/Antineoplastic Agents, Phytogenic; 0/Ceramides; 0/P-Glycoprotein; 0/Polymers; 33069-62-4/Paclitaxel
Comments/Corrections

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