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Atypical CTSK Transcripts and ARNT Transcription Read-Through Into CTSK.
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MedLine Citation:
PMID:  18629217     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The aryl hydrocarbon receptor nuclear translocator (ARNT) and cathepsin K (CTSK) genes lie in a tandem head-to-tail arrangement on human chromosome 1. The two genes are in extremely close proximity; the usual CTSK transcription start site is less than 1.4 kb downstream of the end of the longest reported ARNT transcript. By generating an RT-PCR product that overlaps both the 3' end of ARNT and the 5' end of CTSK, we show that ARNT transcripts may extend through the ARNT-CTSK intergenic region and progress into the CTSK gene. Furthermore, by using quantitative RT-PCR from several tissues to detect the ARNT expression signature in CTSK introns, we show that ARNT transcripts can read through into CTSK as far as CTSK intron 3, extending approximately 3.7 kb downstream of the end of the longest previously described ARNT mRNA. Given that ARNT and CTSK are expressed in an overlapping range of tissues, ARNT read-through may have a negative impact on CTSK transcript levels by interfering with CTSK expression. We also present evidence for novel CTSK transcripts following sequence analysis of CTSK-derived ESTs and RT-PCR products. These transcripts show alternate 5' splicing and or 5' extension and are sometimes initiated from a cryptic alternative promoter which is upstream of the known CTSK promoter and possibly in the 3' UTR of ARNT.
Authors:
Fabienne S Giraudeau; Jean-Philippe Walhin; Paul R Murdock; Nigel K Spurr; Ian C Gray
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Comparative and functional genomics     Volume:  6     ISSN:  1531-6912     ISO Abbreviation:  Comp. Funct. Genomics     Publication Date:  2005  
Date Detail:
Created Date:  2008-07-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101090797     Medline TA:  Comp Funct Genomics     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  268-76     Citation Subset:  -    
Affiliation:
Department of Discovery Genetics GlaxoSmithKline Research and Development New Frontiers Science Park Harlow UK.
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Full Text
Journal Information
Journal ID (nlm-ta): Comp Funct Genomics
ISSN: 1531-6912
ISSN: 1532-6268
Publisher: Hindawi Publishing Corporation
Article Information
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Copyright ? 2005 Hindawi Publishing Corporation.
open-access: This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received Day: 7 Month: 11 Year: 2004
Revision Received Day: 18 Month: 4 Year: 2005
Accepted Day: 9 Month: 5 Year: 2005
Print publication date: Season: Jul?Aug Year: 2005
Volume: 6 Issue: 5-6
First Page: 268 Last Page: 276
ID: 2447513
PubMed Id: 18629217
Publisher Item Identifier: S1531691205000367
DOI: 10.1002/cfg.483

Atypical CTSK Transcripts and ARNT Transcription Read-Through Into CTSK
Fabienne S. Giraudeau1
Jean-Philippe Walhin2
Paul R. Murdock2
Nigel K. Spurr1
Ian C. Gray13 Address: igray@paradigmtherapeutics.com
1Department of Discovery GeneticsGlaxoSmithKline Research and DevelopmentNew Frontiers Science ParkHarlowUK
2Department of Cellular GenomicsGlaxoSmithKline Research and DevelopmentGunnels Wood RoadStevenageUK
3Paradigm Therapeutics (S) Pte Ltd10 Biopolis Road#03-01 ChromosSingapore


Article Categories:
  • Research Article


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