Document Detail


Attenuation of oxidative stress-induced changes in thalassemic erythrocytes by vitamin E.
MedLine Citation:
PMID:  15047982     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oxidative stress status of the transfusion-dependent Ebeta- and beta-thalassemia patients were studied before and after treatment with vitamin E for a period of four weeks. The level of cellular vitamin antioxidants viz. ascorbic acid and vitamin E in the thalassemia patients were found to be considerably lower compared to normal subjects. The activities of enzymatic antioxidants viz. catalase, glutathione peroxidase and glutathione reductase were found to be drastically reduced in untreated Ebeta- and beta-thalassemic patients when compared to normal subjects. However, the activity of superoxide dis-mutase was found to be increased in both types of untreated thalassemic patients when compared to normal individuals. An increase in superoxide dismutase and a decrease in catalase activity reflects the presence of a severe oxidative stress situation in the erythrocytes of the untreated transfusion dependent Ebeta- and beta-thalassemia patients. Changes in erythrocyte membrane protein pattern in untreated Ebeta- and beta-thalassemia patients when compared to normal erythrocyte further confirm the presence of continued oxidative stress in the ailing thalassemic erythrocytes. All these changes in the antioxidant status as well as the changes in the erythrocyte membrane proteins are ameliorated to considerable extent when the transfusion-dependent Ebeta- and beta-thalassemia patients were treated with vitamin E at a dose of 10 mg/kg/day for a period of four weeks. The patients during the treatment period did not exhibit any side effects and gained in body weight indicating a healthy status. The present study reveals that the lipophilic antioxidant vitamin E could be useful in the management of transfusion-dependant Ebeta- and beta-thalassemia patients.
Authors:
Nandita Das; Tapasi Das Chowdhury; Aindrila Chattopadhyay; Asoke G Datta
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Polish journal of pharmacology     Volume:  56     ISSN:  1230-6002     ISO Abbreviation:  Pol J Pharmacol     Publication Date:    2004 Jan-Feb
Date Detail:
Created Date:  2004-03-29     Completed Date:  2005-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313882     Medline TA:  Pol J Pharmacol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  85-96     Citation Subset:  IM    
Affiliation:
Department of Life Science and Biotechnology, Jadavpur University, Kolkata-700 032, India.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Ascorbic Acid / blood
Blood Transfusion
Catalase / drug effects,  metabolism
Child
Child, Preschool
Drug Administration Schedule
Electrophoresis, Polyacrylamide Gel / methods
Erythrocyte Membrane / chemistry,  drug effects,  metabolism
Female
Glutathione Peroxidase / metabolism
Glutathione Reductase / metabolism
Hemoglobin E / chemistry,  drug effects
Humans
India
Lipid Peroxidation / drug effects
Male
Oxidative Stress / drug effects*,  physiology
Superoxide Dismutase / drug effects,  metabolism
Time Factors
Tyrosine / metabolism
Vitamin E / blood,  pharmacology,  therapeutic use*
beta-Thalassemia / blood*,  classification,  drug therapy*
Chemical
Reg. No./Substance:
1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 55520-40-6/Tyrosine; 9034-61-1/Hemoglobin E; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase; EC 1.8.1.7/Glutathione Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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