| Attenuation of oxidant-mediated endothelial cell injury with docosahexaenoic acid: the role of intracellular iron. | |
| | |
MedLine Citation:
|
PMID: 9774176 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Previous studies have demonstrated that altering the fatty acid composition of porcine pulmonary artery endothelial cells (PAEC) significantly modulates their susceptibility to oxidative stimuli, e.g. H2O2. Based on observations that fatty acids also function to transport iron, an important catalyst for H2O2-mediated hydroxyl radical generation, we hypothesized that fatty acid-induced alterations in PAEC iron metabolism contribute to modulation of PAEC oxidant susceptibility. To test this hypothesis, PAEC were treated with culture medium supplemented with 0.1 mM oleic (18:1), linolenic (18:3) or docosahexaenoic (22:6) acids or with an equivalent volume of ethanol vehicle for 3 h. After thorough washing and incubation in unsupplemented culture medium for 24 h, PAEC monolayers were subjected to additional studies. Supplementation with 22:6 attenuated lactate dehydrogenase (LDH) release from PAEC 2 h following treatment with 100 microM H2O2 for 30 min (% LDH release: ETOH-control = 7.9 +/- 1.6, 22:6-control = 5.9 +/- 0.9, ETOH-H2O2 = 26.4 +/- 4.2, 22:6-H2O2* = 16.2 +/- 2.9; *P < 0.05 vs ETOH-H2O2). In a non-cellular system, 18:1 and 18:3 were more effective than their methyl ester derivatives or 22:6 at translocating iron from aqueous to hydrophobic environments. In contrast, only supplementation with 22:6 significantly increased PAEC uptake of 57Fe and human umbilical vein endothelial cell (HUVEC) ferritin content, whereas none of the supplementation conditions altered PAEC catalytic iron measured with bleomycin. These novel observations indicate that specific fatty acids are capable of altering PAEC iron uptake and ferritin content thereby contributing to the understanding of the mechanisms by which fatty acids modulate the oxidant susceptibility of vascular endothelial cells. |
| | |
Authors:
|
M D Ober; C M Hart |
Related Documents
:
|
18960586 - Solvent extraction-absorptiometric determination of niobium in steels with bromopyrogal... 21775116 - Trans-10, cis-12 conjugated linoleic acid decreases de novo lipid synthesis in human ad... 8648596 - Synthesis and biological evaluation of naphthyldesferrithiocin iron chelators. 1904486 - Influence of calcium and amino acids on the osmium impregnation of the endoplasmic reti... 23652896 - Protective effects of a topical antioxidant complex containing vitamins c and e and fer... 8258526 - Protection of retinal pigment epithelium from oxidative injury by glutathione and precu... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Prostaglandins, leukotrienes, and essential fatty acids Volume: 59 ISSN: 0952-3278 ISO Abbreviation: Prostaglandins Leukot. Essent. Fatty Acids Publication Date: 1998 Aug |
Date Detail:
|
Created Date: 1998-12-09 Completed Date: 1998-12-09 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8802730 Medline TA: Prostaglandins Leukot Essent Fatty Acids Country: SCOTLAND |
Other Details:
|
Languages: eng Pagination: 127-35 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Indiana University and Richard L. Roudebush Veterans Affairs Medical Centers, Indianapolis, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bleomycin / pharmacology Cell Survival / drug effects Cell-Free System Cells, Cultured Chelating Agents / pharmacology Deferoxamine / pharmacology Docosahexaenoic Acids / pharmacology* Endothelium, Vascular / cytology*, drug effects, metabolism Ferritins / metabolism Humans Hydrogen Peroxide / toxicity Iron / metabolism* L-Lactate Dehydrogenase / metabolism Linoleic Acid / pharmacology Oleic Acid / pharmacology Oxidants / toxicity* Pulmonary Artery Solubility Swine Umbilical Veins |
| Chemical | |
Reg. No./Substance:
|
0/Chelating Agents; 0/Oxidants; 11056-06-7/Bleomycin; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; 25167-62-8/Docosahexaenoic Acids; 70-51-9/Deferoxamine; 7439-89-6/Iron; 7722-84-1/Hydrogen Peroxide; 9007-73-2/Ferritins; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Helicobacter pylori fatty acid cis 9,10-methyleneoctadecanoic acid increases [Ca2+]i, activates prot...
Next Document: Hypoxic pulmonary vasoconstriction in pigs: role of endothelin-1, prostanoids and ATP-dependent pota...