Document Detail


Attenuation of oxidant damage in the post-conditioned heart involves non-enzymatic response and partial catalytic protection.
MedLine Citation:
PMID:  22562812     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Oxidant stress among other effectors, are implicated in the sequel of myocardial reperfusion injury; thereby it is accepted that maintaining the balance between oxidant/antioxidant signaling within the cell will provide protection against reperfusion damage. The cardioprotective strategy of post-conditioning (PC) reduces reperfusion injury through complex mechanisms; however the contribution of the antioxidant system has not been fully studied. In this work isolated rat hearts were subjected to 30-min global ischemia, PC and 5-min (IR5) or 60-min (IR60) of reperfusion. PC significantly increased the left ventricular developed pressure (LVDP) and the double product (DP: heart rate x LVDP) at both early (PC5) and long reperfusion (PC60). Necrotic tissue diminished to 10.8% in PC60 hearts as compared with 49% of infarct size measured in IR60 hearts (P < 0.05 vs. IR60); also protein carbonylation and malondialdehyde levels decreased in correlation with a significant augment in CuZn superoxide dismutase (CuZnSOD) activity (P < 0.05, PC60 vs. IR60) and increased glutathione redox state (GSH: GSSG) (P < 0.05 PC60 vs. IR60). Diethylthiocarbamate (DETC), a non-selective SOD inhibitor, significantly diminished PC-protection when administrated during all the protocol. However, administration of this inhibitor only during reperfusion had no effect on PC-induced cardioprotection. These results indicate that non-enzymatic antioxidants account for the protective effect of PC, modifying the oxidant stress caused by ischemic reperfusion in rats. The contribution of CuZnSOD activity in the observed cardioprotective effect is less clear, and could be relevant if acting in concert with other PC-activated mechanisms.
Authors:
Francisco Arreguín; Noemí García; Sauri Hernández-Reséndiz; Mabel Buelna-Chontal; Francisco Correa; Viridiana Olín-Sandoval; Omar Noel Medina-Campos; José Pedraza-Chaverri; Cecilia Zazueta
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-4
Journal Detail:
Title:  Experimental physiology     Volume:  -     ISSN:  1469-445X     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 Instituto Nacional de Cardiología Ignacio Chávez;
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