Document Detail

Attenuation and laboratory markers of the Oka-strain varicella-zoster virus.
MedLine Citation:
PMID:  3014476     Owner:  NLM     Status:  MEDLINE    
A live varicella vaccine was developed by serial passages of the Oka-strain varicella-zoster virus (VZV), isolated in our laboratory, in human embryonic lung cells and guinea-pig embryonic fibroblasts (GPEF). It is slightly temperature sensitive at 39 degrees C and shows a higher ratio of infectivity in GPEF to that in human embryonic fibroblasts (HuEF) than wild-type strains, indicating that it is a variant in thermosensitivity and host dependency. A DNA digest with the HpaI enzyme of the Oka strain contained a unique fragment, denoted K. Clinical studies with VZV isolated from vaccinees indicated that the GPEF/HuEF ratio of infectivity and the profiles of HpaI DNA digests are useful tests to differentiate the vaccine from wild-type strains. Prompt vaccination of household contacts proved effective in preventing spread of clinical varicella and seemed to be related to the early appearance (4-7 days after vaccination) of cell-mediated immunity in vaccinees, assessed by the VZV skin test. VZV could be isolated from mononuclear blood cells of varicella patients shortly before or after appearance of rashes, whereas no virus could be detected in 27 vaccinees 4-14 days after vaccination, suggesting that replication of the vaccine virus in susceptible organs and blood cells in humans is far less than that of wild-type viruses, but sufficient to induce VZV immunity. This difference seems to be related to the attenuated nature of the vaccine strain.
M Takahashi; Y Hayakawa; K Shiraki; K Yamanishi; Y Asano; T Ozaki
Related Documents :
11747706 - Expanding global distribution of rotavirus serotype g9: detection in libya, kenya, and ...
6330386 - Live attenuated varicella vaccine. efficacy for children with leukemia in remission.
17596796 - Immunogenicity of oka/merck varicella vaccine in children vaccinated at 12-14 months of...
18998756 - Sixteen years of global experience with the first refrigerator-stable varicella vaccine...
17651156 - Intranasal delivery of whole influenza vaccine prevents subsequent allergen-induced sen...
2601226 - Development of babesia ovata in the midgut of the tick, haemaphysalis longicornis.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Postgraduate medical journal     Volume:  61 Suppl 4     ISSN:  0032-5473     ISO Abbreviation:  Postgrad Med J     Publication Date:  1985  
Date Detail:
Created Date:  1986-07-25     Completed Date:  1986-07-25     Revised Date:  2009-10-22    
Medline Journal Info:
Nlm Unique ID:  0234135     Medline TA:  Postgrad Med J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  37-46     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chickenpox / prevention & control*
Chickenpox Vaccine
Child, Preschool
Fibroblasts / microbiology
Guinea Pigs
Herpesvirus 3, Human / immunology*,  physiology
Lung / embryology,  microbiology
Plaque Assay
Vaccines, Attenuated
Viral Vaccines*
Reg. No./Substance:
0/Chickenpox Vaccine; 0/Vaccines, Attenuated; 0/Viral Vaccines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Management of varicella-zoster contact and infection in pregnancy using a standardized varicella-zos...
Next Document:  Laboratory markers of immunity to varicella-zoster virus.