Document Detail


Attenuation and laboratory markers of the Oka-strain varicella-zoster virus.
MedLine Citation:
PMID:  3014476     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A live varicella vaccine was developed by serial passages of the Oka-strain varicella-zoster virus (VZV), isolated in our laboratory, in human embryonic lung cells and guinea-pig embryonic fibroblasts (GPEF). It is slightly temperature sensitive at 39 degrees C and shows a higher ratio of infectivity in GPEF to that in human embryonic fibroblasts (HuEF) than wild-type strains, indicating that it is a variant in thermosensitivity and host dependency. A DNA digest with the HpaI enzyme of the Oka strain contained a unique fragment, denoted K. Clinical studies with VZV isolated from vaccinees indicated that the GPEF/HuEF ratio of infectivity and the profiles of HpaI DNA digests are useful tests to differentiate the vaccine from wild-type strains. Prompt vaccination of household contacts proved effective in preventing spread of clinical varicella and seemed to be related to the early appearance (4-7 days after vaccination) of cell-mediated immunity in vaccinees, assessed by the VZV skin test. VZV could be isolated from mononuclear blood cells of varicella patients shortly before or after appearance of rashes, whereas no virus could be detected in 27 vaccinees 4-14 days after vaccination, suggesting that replication of the vaccine virus in susceptible organs and blood cells in humans is far less than that of wild-type viruses, but sufficient to induce VZV immunity. This difference seems to be related to the attenuated nature of the vaccine strain.
Authors:
M Takahashi; Y Hayakawa; K Shiraki; K Yamanishi; Y Asano; T Ozaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Postgraduate medical journal     Volume:  61 Suppl 4     ISSN:  0032-5473     ISO Abbreviation:  Postgrad Med J     Publication Date:  1985  
Date Detail:
Created Date:  1986-07-25     Completed Date:  1986-07-25     Revised Date:  2009-10-22    
Medline Journal Info:
Nlm Unique ID:  0234135     Medline TA:  Postgrad Med J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  37-46     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chickenpox / prevention & control*
Chickenpox Vaccine
Child, Preschool
Fibroblasts / microbiology
Guinea Pigs
Herpesvirus 3, Human / immunology*,  physiology
Humans
Lung / embryology,  microbiology
Male
Plaque Assay
Vaccines, Attenuated
Viral Vaccines*
Chemical
Reg. No./Substance:
0/Chickenpox Vaccine; 0/Vaccines, Attenuated; 0/Viral Vaccines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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