Document Detail


Attenuation of heat shock protein expression by coronary occlusion in hypertrophied hearts.
MedLine Citation:
PMID:  9277465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hearts hypertrophied by pressure-overload are more susceptible to ischemia than nonhypertrophied hearts, which may result from the attenuation of self-protective responses. Because heat shock proteins (HSPs) are reported to protect against ischemic injuries, we hypothesized that HSP expression by coronary occlusion may be attenuated in hypertrophied hearts. We banded the ascending aorta to develop ventricular hypertrophy and put a snare around the left coronary artery in rats. After 4 wk, coronary occlusion was applied by tightening the snare for 5 or 10 min in rats with and without aortic banding. The hearts were excised 0, 0.5, 1, 2, 4, 8, 12, and 24 h after coronary occlusion. Ischemic and nonischemic myocardial tissues were obtained after the snare was tightly tied, and dye was infused from the aorta. The mRNAs and protein of 72-kDa HSP (HSP 72) and/or 73-kDa HSP (HSP 73) were detected by Northern and Western blot analyses. Protein and mRNA levels of HSPs expressed by 5-min coronary occlusion in hypertrophied hearts (left ventricular weight, 577 +/- 16 mg) were lower compared with those in control hearts (462 +/- 9 mg). A longer period of coronary occlusion (10 min) elevated the attenuated expression to a level similar to that in control hearts. Treatment with an angiotensin-converting enzyme (ACE) inhibitor (cilazapril, 10-15 mg.kg(-1).day(-1)) for 4 wk preserved HSP mRNA expression even in hearts with ascending aortic banding. In hypertrophied hearts, HSP 72 and 73 expression by coronary occlusion was attenuated and was modulated by the duration of coronary occlusion and by ACE inhibitor treatment.
Authors:
M Tajima; S Isoyama; Y Nitta; K Abe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  273     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-09-25     Completed Date:  1997-09-25     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H526-33     Citation Subset:  IM    
Affiliation:
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
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MeSH Terms
Descriptor/Qualifier:
Angiotensin-Converting Enzyme Inhibitors / pharmacology
Animals
Body Weight
Cardiomegaly / metabolism*,  pathology
Carrier Proteins / genetics,  metabolism*
Cilazapril / pharmacology
Coronary Disease / metabolism*,  pathology
HSC70 Heat-Shock Proteins
HSP70 Heat-Shock Proteins*
HSP72 Heat-Shock Proteins
Heat-Shock Proteins / genetics,  metabolism*
Male
Myocardium / metabolism*,  pathology
Organ Size
RNA, Messenger / metabolism
Rats
Rats, Wistar
Time Factors
Chemical
Reg. No./Substance:
0/Angiotensin-Converting Enzyme Inhibitors; 0/Carrier Proteins; 0/HSC70 Heat-Shock Proteins; 0/HSP70 Heat-Shock Proteins; 0/HSP72 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Hspa8 protein, rat; 0/RNA, Messenger; 92077-78-6/Cilazapril

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