| Attenuation of breast tumor cell growth by conjugated linoleic acid via inhibition of 5-lipoxygenase activating protein. | |
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MedLine Citation:
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PMID: 16185917 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Conjugated linoleic acid (CLA) consists of a group of linoleic acid geometric isomers that have been shown to reduce tumor growth and metastasis in animal models of breast, prostate and colon cancer. To delineate a possible mechanism of action for CLA, we have recently shown that the 5-lipoxygenase product, 5-hydroxyeicosatetraenoic acid (5-HETE), could play a role in CLA alteration of mammary tumorigenesis. In this study, we determined how CLA could modulate 5-lipoxygenase activity. The t10, c12-CLA isomer reduced production of 5-HETE but not 12- and 15-HETE in MDA-MB-231 human breast tumor cells. That isomer and the c9, t11-CLA isomer decreased 5-HETE production by competition with the lipoxygenase substrate, arachidonic acid (AA). Interestingly, t10, c12-CLA reduced the expression of five-lipoxygenase activating protein (FLAP) but not the 5-lipoxygenase enzyme. Over-expression of FLAP abrogated t10, c12-CLA-reduced viability of MDA-MB-231 cells. These data suggest that the reduction of 5-HETE by t10, c12-CLA was due to competition with AA and the reduction of FLAP expression. |
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Authors:
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Jung-Hyun Kim; Neil E Hubbard; Vincent Ziboh; Kent L Erickson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1736 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-10 Completed Date: 2005-12-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 244-50 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Human Anatomy, University of California, School of Medicine, Davis, CA 95616-8643, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
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metabolism Arachidonate 5-Lipoxygenase / genetics, metabolism Arachidonic Acid / pharmacology Breast Neoplasms / genetics, metabolism, pathology Carrier Proteins / genetics* Cell Line, Tumor Cell Proliferation / drug effects* Cell Survival / drug effects, genetics Female Gene Expression / drug effects Humans Hydroxyeicosatetraenoic Acids / metabolism, pharmacology Isomerism Linoleic Acids / metabolism Linoleic Acids, Conjugated / pharmacology* Membrane Proteins / genetics* Transfection |
| Chemical | |
Reg. No./Substance:
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0/5-lipoxygenase-activating protein; 0/Carrier Proteins; 0/Hydroxyeicosatetraenoic Acids; 0/Linoleic Acids; 0/Linoleic Acids, Conjugated; 0/Membrane Proteins; 506-32-1/Arachidonic Acid; 5204-88-6/13-hydroxy-9,11-octadecadienoic acid; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 72255-35-7/5-hydroxy-6,8,11,14-eicosatetraenoic acid; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid; EC 1.13.11.34/Arachidonate 5-Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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