Document Detail

Attenuation of anti-Thy1 glomerulonephritis in the rat by anti-inflammatory platelet-inhibiting agents.
MedLine Citation:
PMID:  7679550     Owner:  NLM     Status:  MEDLINE    
Although both ecto-ADPase and prostacyclin (PGI2) inhibit platelets and neutrophils, their action in acute glomerulonephritis is unknown. We tested the PGI2 analog Iloprost and 2chloroadenosine (2Cl-ADO), an analog of adenosine, the end product of nucleotidase activities, during anti-Thy1 nephritis. Rats received anti-Thy1 immunoglobulin G (5 mg/kg body weight, intravenously) and subsequently one subcutaneous injection of either 2Cl-ADO (10 mg/kg body weight; (n = 6) or Iloprost (1 mg/kg body weight; n = 6). Control rats received anti-Thy1 immunoglobulin G with saline (n = 6) or saline alone (n = 6). After 24 hours, kidneys were processed for light-microscopical evaluation. Proteinuria was studied in additional rats. Results showed that both drugs inhibited intraglomerular platelet activation (P < 0.005). 2Cl-ADO also reduced intraglomerular O2- production of neutrophils (P < 0.05), in contrast to Iloprost. Intraglomerular immunoglobulin G deposition, complement activation, neutrophil influx, and myeloperoxidase release were not affected by 2Cl-ADO or Iloprost. However, proteinuria was completely prevented by both drugs. It is concluded that PGI2 and nucleotidases are potentially able to attenuate this form of nephritis by inhibiting platelet activity, whereas nucleotidases also inhibit neutrophil activity in vivo.
K Poelstra; E Brouwer; J F Baller; M J Hardonk; W W Bakker
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of pathology     Volume:  142     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-16     Completed Date:  1993-03-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  441-50     Citation Subset:  AIM; IM    
Department of Pathology, University of Groningen, The Netherlands.
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MeSH Terms
2-Chloroadenosine / pharmacology
Exudates and Transudates / cytology,  metabolism
Glomerulonephritis / immunology*,  metabolism,  prevention & control
Iloprost / pharmacology
Immunoglobulin G / immunology
Immunohistochemistry / methods
Isoantibodies / immunology*
Kidney Glomerulus / metabolism,  pathology
Oxygen / metabolism
Peritoneal Cavity / pathology
Peroxidase / metabolism
Platelet Aggregation Inhibitors / pharmacology*
Proteinuria / etiology
Rats, Inbred Strains
Staining and Labeling
Reg. No./Substance:
0/Immunoglobulin G; 0/Isoantibodies; 0/Platelet Aggregation Inhibitors; 0/anti-Thy antibody; 146-77-0/2-Chloroadenosine; 7782-44-7/Oxygen; 78919-13-8/Iloprost; EC

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