Document Detail

Attenuation of adipocyte triacylglycerol hydrolase activity decreases basal fatty acid efflux.
MedLine Citation:
PMID:  17237500     Owner:  NLM     Status:  MEDLINE    
Fatty acids released from adipose triacylglycerol stores by lipolysis provide vertebrates with an important source of energy. We investigated the role of microsomal triacylglycerol hydrolase (TGH) in the mobilization of adipocyte triacylglycerols through inactivation of the TGH activity by RNA interference or chemical inhibition. Attenuation of TGH activity resulted in decreased basal but not isoproterenol-stimulated efflux of fatty acids from 3T3-L1 adipocytes. Lack of TGH activity was accompanied by accumulation of cellular triacylglycerols and cholesteryl esters without any changes in the expression of enzymes catalyzing triacylglycerol synthesis (diacylglycerol acyltransferases 1 and 2) or degradation (adipose triglyceride lipase and hormone-sensitive lipase). Inhibition of TGH-mediated lipolysis also did not affect insulin-stimulated Glut4 translocation from intracellular compartments to the plasma membrane or glucose uptake into adipocytes. These data suggest that TGH plays a role in adipose tissue triacylglycerol metabolism and may be a suitable pharmacological target for lowering fatty acid efflux from adipose tissue without altering glucose import.
Enhui Wei; Wenhui Gao; Richard Lehner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-21
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  282     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-12     Completed Date:  2007-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8027-35     Citation Subset:  IM    
Department of Pediatrics, Canadian Institutes of Health Research Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
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MeSH Terms
3T3-L1 Cells
Adipocytes / enzymology*,  metabolism
Adipose Tissue / metabolism
Biological Transport
Cell Membrane / metabolism
Fatty Acids / metabolism*
Fibroblasts / metabolism
Glucose / metabolism
Glucose Transporter Type 4 / metabolism
Insulin / metabolism
Lipase / metabolism*
Microsomes / enzymology*
Triglycerides / metabolism
Reg. No./Substance:
0/Fatty Acids; 0/Glucose Transporter Type 4; 0/Triglycerides; 11061-68-0/Insulin; 50-99-7/Glucose; EC

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