Document Detail


Attenuation of MG132-induced HeLa cell death by N-acetyl cysteine via reducing reactive oxygen species and preventing glutathione depletion.
MedLine Citation:
PMID:  20651358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MG132 as a proteasome inhibitor can induce apoptotic cell death through formation of reactive oxygen species (ROS). In this study, the effects of N-acetyl cysteine (NAC; an antioxidant) on MG132-induced HeLa cell death in relation to ROS and glutathione (GSH) were investigated. MG132 induced cell growth inhibition and apoptosis in HeLa cells, which was accompanied by the loss of mitochondrial membrane potential (MMP; Delta Psi(m)), activation of caspase-3 and poly (ADP-ribose) polymerase (PARP) cleavage. MG132 increased ROS levels, including O(2)(*-), and GSH depleted cell numbers of HeLa cells. NAC reduced the number of annexin V-positive cells and MMP (Delta Psi(m)) loss by MG132. In addition, NAC significantly reduced the ROS level and prevented GSH depletion. In conclusion, NAC prevented MG132-induced HeLa cell death via decreasing ROS and preventing GSH depletion.
Authors:
Yong Hwan Han; Yeon Mi Yang; Sung Zoo Kim; Woo Hyun Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-08-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2107-12     Citation Subset:  IM    
Affiliation:
Department of Physiology, Medical School, Chonbuk National University, JeonJu, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Caspase 3 / physiology
Cell Cycle / drug effects
Cell Proliferation / drug effects
Glutathione / metabolism*
Hela Cells
Humans
Leupeptins / pharmacology*
Membrane Potential, Mitochondrial / drug effects
Proteasome Endopeptidase Complex / antagonists & inhibitors
Reactive Oxygen Species / metabolism*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Leupeptins; 0/Reactive Oxygen Species; 133407-82-6/benzyloxycarbonylleucyl-leucyl-leucine aldehyde; 616-91-1/Acetylcysteine; 70-18-8/Glutathione; EC 3.4.22.-/Caspase 3; EC 3.4.25.1/Proteasome Endopeptidase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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